{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T07:40:13Z","timestamp":1790754013243,"version":"4.1.0"},"reference-count":89,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,8,31]],"date-time":"2019-08-31T00:00:00Z","timestamp":1567209600000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2018,8,31]],"date-time":"2018-08-31T00:00:00Z","timestamp":1535673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["AGL2015\u201074071\u2010JIN"],"award-info":[{"award-number":["AGL2015\u201074071\u2010JIN"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["AGL2016\u201077267\u2010R"],"award-info":[{"award-number":["AGL2016\u201077267\u2010R"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DBI\u2010C421683"],"award-info":[{"award-number":["DBI\u2010C421683"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["RCN009281"],"award-info":[{"award-number":["RCN009281"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["La606\/13\u20101"],"award-info":[{"award-number":["La606\/13\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>\n<jats:list list-type=\"bullet\">\n\n<jats:list-item><jats:p>In angiosperms, pollen tube entry into the ovule generally takes place through the micropyle, but the exact role of the micropyle in pollen tube guidance remains unclear. A limited number of studies have examined eudicots with bitegmic micropyles, but information is lacking in ovules of basal\/early\u2010divergent angiosperms with unitegmic micropyles.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>We have evaluated the role of the micropyle in pollen tube guidance in an early\u2010divergent angiosperm (<jats:italic>Annona cherimola<\/jats:italic>) and the evolutionarily derived <jats:italic>Arabidopsis thaliana<\/jats:italic> by studying \u03b3\u2010aminobutyric acid (<jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content>) and arabinogalactan proteins (<jats:styled-content style=\"fixed-case\">AGP<\/jats:styled-content>s) in wild\u2010type plants and integument\u2010defective mutants.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>A conserved inhibitory role of <jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content> in pollen tube growth was shown in <jats:italic>A.\u00a0cherimola<\/jats:italic>, in which <jats:styled-content style=\"fixed-case\">AGP<\/jats:styled-content>s surround the egg apparatus. In <jats:italic>Arabidopsis<\/jats:italic>, the micropyle formed only by the outer integument in <jats:italic>wuschel\u20107<\/jats:italic> mutants caused a partial defect in pollen tube guidance. Moreover, pollen tubes were not observed in the micropyle of an <jats:italic>inner no outer<\/jats:italic> (<jats:italic>ino<\/jats:italic>) mutant in <jats:italic>Arabidopsis<\/jats:italic>, but were observed in homologous <jats:italic>ino<\/jats:italic> mutants in <jats:italic>Annona<\/jats:italic>.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>The similar distribution of <jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">AGP<\/jats:styled-content>s observed in the micropyle of <jats:italic>Arabidopsis<\/jats:italic> and <jats:italic>Annona<\/jats:italic>, together with the anomalies from specific integument mutants, support the role of the inner integument in preventing multiple tube entrance (polytubey) in these two phylogenetically distant genera.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.15420","type":"journal-article","created":{"date-parts":[[2018,8,31]],"date-time":"2018-08-31T18:36:36Z","timestamp":1535740596000},"page":"1074-1089","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["The role of the integuments in pollen tube guidance in flowering plants"],"prefix":"10.1111","volume":"221","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9713-0431","authenticated-orcid":false,"given":"Jorge","family":"Lora","sequence":"first","affiliation":[{"name":"Department of Subtropical Fruit Crops Instituto de Hortofruticultura Subtropical y Mediterr\u00e1nea \u2018La Mayora\u2019 (IHSM\u2010UMA\u2010CSIC) 29750 Algarrobo\u2010Costa, M\u00e1laga Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Laux","sequence":"additional","affiliation":[{"name":"BIOSS Centre for Biological Signalling Studies Faculty of Biology University of Freiburg Sch\u00e4nzlestrasse 1 79104 Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5449-7444","authenticated-orcid":false,"given":"Jos\u00e9 I.","family":"Hormaza","sequence":"additional","affiliation":[{"name":"Department of Subtropical Fruit Crops Instituto de Hortofruticultura Subtropical y Mediterr\u00e1nea \u2018La Mayora\u2019 (IHSM\u2010UMA\u2010CSIC) 29750 Algarrobo\u2010Costa, M\u00e1laga Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/boj.12385"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aob.a087500"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.1991.tb00992.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/145.4.1109"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00497-011-0162-3"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90427-1"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.7.3358"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erm259"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02539808"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1537-2197.1957.tb10567.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.015800"},{"key":"e_1_2_7_13_1","first-page":"155","article-title":"AINTEGUMENTA, an APETALA2\u2010like gene of Arabidopsis with pleiotropic roles in ovule development and floral organ growth","volume":"8","author":"Elliott RC","year":"1996","journal-title":"Plant Cell"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.110.156000"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.3417\/2009139"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcr120"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1143562"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.7.3.333"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.49.1.1"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3732\/ajb.94.6.1046"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00497-009-0094-3"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1810607"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1537-2197.1996.tb12690.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.225202"},{"key":"e_1_2_7_25_1","first-page":"e56441","article-title":"Whole\u2010mount clearing and staining of Arabidopsis flower organs and siliques","volume":"134","author":"Hedhly A","year":"2018","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00024429"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004970100082"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1537-2197.1989.tb15124.x"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.36.1.1"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aob.a085414"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.16.01571"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2008.02457.x"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00497-002-0143-7"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.7.1.57"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00196566"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.105.034603"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1991.t01-9-00999.x"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.1.87"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00709-010-0231-8"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.2307\/3869758"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2011.05.015"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1002139"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00629340"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.3732\/ajb.1000185"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.14330"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcp276"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.13460"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.00107"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1014514108"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scienta.2005.12.003"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-9-129"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcs116"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-14-1"},{"key":"e_1_2_7_54_1","first-page":"989","article-title":"Pollen tube access to the ovule is mediated by glycoprotein secretion on the obturator of apple (Malus\u00a0\u00d7\u00a0domestica, Borkh)","volume":"119","author":"Losada JM","year":"2017","journal-title":"Annals of Botany"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.3732\/ajb.1400280"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.3732\/ajb.1700319"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.122.1.3"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1104954"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.03.018"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2005.02588.x"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2006.01875.x"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2016.02.040"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007090200009"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-014-1690-8"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07882"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00479-3"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.2307\/3869311"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.01895"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2016.01.002"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcr021"},{"key":"e_1_2_7_71_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00709-007-0245-z"},{"key":"e_1_2_7_72_1","doi-asserted-by":"publisher","DOI":"10.2307\/3869410"},{"key":"e_1_2_7_73_1","first-page":"335","article-title":"TOUSLED participates in apical tissue formation during gynoecium development in Arabidopsis","volume":"9","author":"Roe JL","year":"1997","journal-title":"Plant Cell"},{"key":"e_1_2_7_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4238(99)00012-6"},{"key":"e_1_2_7_75_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11738-005-0070-y"},{"key":"e_1_2_7_76_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_7_77_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.7.1367"},{"key":"e_1_2_7_78_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1995.07050731.x"},{"key":"e_1_2_7_79_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-9-29"},{"key":"e_1_2_7_80_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.015933"},{"key":"e_1_2_7_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2008.03.014"},{"key":"e_1_2_7_82_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-012-1774-z"},{"key":"e_1_2_7_83_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001449"},{"key":"e_1_2_7_84_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.23.3160"},{"key":"e_1_2_7_85_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5292.1535"},{"key":"e_1_2_7_86_1","doi-asserted-by":"publisher","DOI":"10.1038\/33929"},{"key":"e_1_2_7_87_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90428-X"},{"key":"e_1_2_7_88_1","doi-asserted-by":"publisher","DOI":"10.2307\/3869903"},{"key":"e_1_2_7_89_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/6.2.131"},{"key":"e_1_2_7_90_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-10-36"}],"container-title":["New Phytologist"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnph.15420","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nph.15420","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/nph.15420","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/am-pdf\/10.1111\/nph.15420","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nph.15420","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T11:35:20Z","timestamp":1694259320000},"score":1,"resource":{"primary":{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/10.1111\/nph.15420"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,31]]},"references-count":89,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["10.1111\/nph.15420"],"URL":"https:\/\/doi.org\/10.1111\/nph.15420","archive":["Portico"],"relation":{},"ISSN":["0028-646X","1469-8137"],"issn-type":[{"value":"0028-646X","type":"print"},{"value":"1469-8137","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,31]]},"assertion":[{"value":"2018-05-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}