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Angiosperms carry two\n RPW<\/jats:styled-content>\n 8\u2010\n NLR<\/jats:styled-content>\n subclasses:\n ADR<\/jats:styled-content>\n 1 and\n NRG<\/jats:styled-content>\n 1.\n ADR<\/jats:styled-content>\n 1s act as \u2018helper\u2019\n NLR<\/jats:styled-content>\n s for multiple\n TIR<\/jats:styled-content>\n \u2010 and\n CC<\/jats:styled-content>\n \u2010\n NLR<\/jats:styled-content>\n R proteins in Arabidopsis. In angiosperm families,\n \n NRG<\/jats:styled-content>\n 1\n <\/jats:italic>\n co\u2010occurs with\n \n TIR<\/jats:styled-content>\n \u2010\n NLR<\/jats:styled-content>\n Resistance\n <\/jats:italic>\n (\n R<\/jats:italic>\n ) genes. We tested whether\n NRG<\/jats:styled-content>\n 1 is required for signalling of multiple\n TIR<\/jats:styled-content>\n \u2010\n NLR<\/jats:styled-content>\n s.\n <\/jats:p>\n <\/jats:list-item>\n \n \n Using\n CRISPR<\/jats:styled-content>\n mutagenesis, we obtained an\n nrg1a\u2010nrg1b<\/jats:italic>\n double mutant in two Arabidopsis accessions, and an\n nrg1<\/jats:italic>\n mutant in\n Nicotiana benthamiana<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:list-item>\n \n \n These mutants are compromised in signalling of all\n TIR<\/jats:styled-content>\n \u2010\n NLR<\/jats:styled-content>\n s tested, including\n WRR<\/jats:styled-content>\n 4A,\n WRR<\/jats:styled-content>\n 4B,\n RPP<\/jats:styled-content>\n 1,\n RPP<\/jats:styled-content>\n 2,\n RPP<\/jats:styled-content>\n 4 and the pairs\n RRS<\/jats:styled-content>\n 1\/\n RPS<\/jats:styled-content>\n 4,\n RRS<\/jats:styled-content>\n 1B\/\n RPS<\/jats:styled-content>\n 4B,\n CHS<\/jats:styled-content>\n 1\/\n SOC<\/jats:styled-content>\n 3 and\n CHS<\/jats:styled-content>\n 3\/\n CSA<\/jats:styled-content>\n 1. In Arabidopsis,\n NRG<\/jats:styled-content>\n 1 is required for the hypersensitive cell death response (\n HR<\/jats:styled-content>\n ) and full oomycete resistance, but not for salicylic acid induction or bacterial resistance. By contrast,\n nrg1<\/jats:italic>\n loss of function does not compromise the\n CC<\/jats:styled-content>\n \u2010\n NLR<\/jats:styled-content>\n R proteins\n RPS<\/jats:styled-content>\n 5 and\n MLA<\/jats:styled-content>\n .\n RPM<\/jats:styled-content>\n 1 and\n RPS<\/jats:styled-content>\n 2 (\n CC<\/jats:styled-content>\n \u2010\n NLR<\/jats:styled-content>\n s) function is slightly compromised in an\n nrg1<\/jats:italic>\n mutant. Thus,\n NRG<\/jats:styled-content>\n 1 is required for full\n TIR<\/jats:styled-content>\n \u2010\n NLR<\/jats:styled-content>\n function and contributes to the signalling of some\n CC<\/jats:styled-content>\n \u2010\n NLR<\/jats:styled-content>\n s.\n <\/jats:p>\n <\/jats:list-item>\n \n \n Some\n NRG<\/jats:styled-content>\n 1\u2010dependent R proteins also signal partially via the\n NRG<\/jats:styled-content>\n 1 sister clade,\n ADR<\/jats:styled-content>\n 1. We propose that some\n NLR<\/jats:styled-content>\n s signal via\n NRG<\/jats:styled-content>\n 1 only, some via\n ADR<\/jats:styled-content>\n 1 only and some via both or neither.\n <\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>","DOI":"10.1111\/nph.15659","type":"journal-article","created":{"date-parts":[[2018,12,24]],"date-time":"2018-12-24T11:00:16Z","timestamp":1545649216000},"page":"966-980","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":279,"title":["Diverse\n NLR<\/scp>\n immune receptors activate defence via the\n RPW<\/scp>\n 8\u2010\n NLR NRG<\/scp>\n 1"],"prefix":"10.1111","volume":"222","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2722-0228","authenticated-orcid":false,"given":"Baptiste","family":"Castel","sequence":"first","affiliation":[{"name":"The Sainsbury Laboratory University of East Anglia Norwich Research Park, Colney Lane Norwich NR4 7UH UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0760-1058","authenticated-orcid":false,"given":"Pok\u2010Man","family":"Ngou","sequence":"additional","affiliation":[{"name":"The Sainsbury Laboratory University of East Anglia Norwich Research Park, Colney Lane Norwich NR4 7UH UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3545-3179","authenticated-orcid":false,"given":"Volkan","family":"Cevik","sequence":"additional","affiliation":[{"name":"The Sainsbury Laboratory University of East Anglia Norwich Research Park, Colney Lane Norwich NR4 7UH UK"},{"name":"The Milner Centre for Evolution Department of Biology and Biochemistry University of Bath Bath BA2 7AY UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5171-8061","authenticated-orcid":false,"given":"Amey","family":"Redkar","sequence":"additional","affiliation":[{"name":"The Sainsbury Laboratory University of East Anglia Norwich Research Park, Colney Lane Norwich NR4 7UH UK"},{"name":"Department of Genetics University of C\u00f3rdoba C\u00f3rdoba 14071 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4579-2094","authenticated-orcid":false,"given":"Dae\u2010Sung","family":"Kim","sequence":"additional","affiliation":[{"name":"The Sainsbury Laboratory University of East Anglia Norwich Research Park, Colney Lane Norwich NR4 7UH UK"},{"name":"Department of Plant Sciences College of Life Sciences Wuhan University Wuhan 430072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Yang","sequence":"additional","affiliation":[{"name":"The Sainsbury Laboratory University of East Anglia Norwich Research Park, Colney Lane Norwich NR4 7UH UK"},{"name":"Center for Plant Science Innovation, Beadle Center University of Lincoln\u2010Nebraska Lincoln NE 68588 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3535-6053","authenticated-orcid":false,"given":"Pingtao","family":"Ding","sequence":"additional","affiliation":[{"name":"The Sainsbury Laboratory University of East Anglia Norwich Research Park, Colney Lane Norwich NR4 7UH UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4953-261X","authenticated-orcid":false,"given":"Jonathan D. 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