{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T12:59:53Z","timestamp":1791809993890,"version":"4.3.4"},"reference-count":61,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000921","name":"European Cooperation in Science and Technology","doi-asserted-by":"publisher","award":["FA1101"],"award-info":[{"award-number":["FA1101"]}],"id":[{"id":"10.13039\/501100000921","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>\n                          Saffron crocus (\n                          <jats:italic>Crocus sativus<\/jats:italic>\n                          ) is the source of the most expensive spice of the world, produced from manually harvested stigmas, thus serving as a cash crop for rural communities. However, despite its economic importance, its genome and chromosomes are poorly studied.\n                          <jats:italic>C.\u00a0sativus<\/jats:italic>\n                          is a sterile triploid species harboring eight chromosome triplets, and propagated only as a clonal lineage by corms. Saffron's evolutionary origin, parental species and allo\u2010 or autotriploidy has been a matter of discussion for almost a century.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          We performed a survey sequencing of the saffron genome and selected cytogenetic landmark sequences consisting of major tandem repeats, which we used as probes in comparative multicolor fluorescent\n                          <jats:italic>in\u00a0situ<\/jats:italic>\n                          hybridization (\n                          <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>\n                          ).\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          We tagged 92 chromosomal positions and resolved the chromosomal composition of saffron triplets. By comparative\n                          <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>\n                          of six\n                          <jats:italic>Crocus<\/jats:italic>\n                          species from 11 accessions, we demonstrate that\n                          <jats:italic>C.\u00a0sativus<\/jats:italic>\n                          is an autotriploid hybrid derived from heterogeneous\n                          <jats:italic>Crocus\u00a0cartwrightianus<\/jats:italic>\n                          cytotypes.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          The\n                          <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>\n                          reference karyotype of saffron is crucial for integrating genome sequencing data with chromosomes and for investigating the relationship among\n                          <jats:italic>Crocus<\/jats:italic>\n                          species. We provide an evolutionary model of the saffron emergence; the knowledge of the parental origin offers a route towards the resynthesis of\n                          <jats:italic>C.\u00a0sativus<\/jats:italic>\n                          from\n                          <jats:italic>C.\u00a0cartwrightianus<\/jats:italic>\n                          to broaden saffron's gene pool.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.15715","type":"journal-article","created":{"date-parts":[[2019,1,28]],"date-time":"2019-01-28T22:39:54Z","timestamp":1548715194000},"page":"1965-1980","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Adding color to a century\u2010old enigma: multi\u2010color chromosome identification unravels the autotriploid nature of saffron (\n                    <i>Crocus sativus<\/i>\n                    ) as a hybrid of wild\n                    <i>Crocus cartwrightianus<\/i>\n                    cytotypes"],"prefix":"10.1111","volume":"222","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7259-415X","authenticated-orcid":false,"given":"Thomas","family":"Schmidt","sequence":"first","affiliation":[{"name":"Faculty of Biology Technische Universit\u00e4t Dresden Dresden D\u201001062 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0168-8428","authenticated-orcid":false,"given":"Tony","family":"Heitkam","sequence":"additional","affiliation":[{"name":"Faculty of Biology Technische Universit\u00e4t Dresden Dresden D\u201001062 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Liedtke","sequence":"additional","affiliation":[{"name":"Faculty of Biology Technische Universit\u00e4t Dresden Dresden D\u201001062 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3072-0485","authenticated-orcid":false,"given":"Veit","family":"Schubert","sequence":"additional","affiliation":[{"name":"Leibniz Institute of Plant Genetics and Crop Plant Research Corrensstr. 3 Seeland D\u201006466 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8912-5162","authenticated-orcid":false,"given":"Gerhard","family":"Menzel","sequence":"additional","affiliation":[{"name":"Faculty of Biology Technische Universit\u00e4t Dresden Dresden D\u201001062 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