{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T07:26:13Z","timestamp":1788247573205,"version":"build-2803163510"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,2,12]],"date-time":"2020-02-12T00:00:00Z","timestamp":1581465600000},"content-version":"am","delay-in-days":331,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2019,3,18]],"date-time":"2019-03-18T00:00:00Z","timestamp":1552867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006151","name":"Basic Energy Sciences","doi-asserted-by":"publisher","award":["DE\u2010FG02\u201007ER15905"],"award-info":[{"award-number":["DE\u2010FG02\u201007ER15905"]}],"id":[{"id":"10.13039\/100006151","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>\n<jats:list list-type=\"bullet\">\n\n<jats:list-item><jats:p>The Mediator complex functions as a hub for transcriptional regulation. <jats:styled-content style=\"fixed-case\">MED<\/jats:styled-content>5, an Arabidopsis Mediator tail subunit, is required for maintaining phenylpropanoid homeostasis. A semidominant mutation (<jats:italic>ref4\u20103<\/jats:italic>) that causes a single amino acid substitution in <jats:styled-content style=\"fixed-case\">MED<\/jats:styled-content>5b functions as a strong suppressor of the pathway, leading to decreased soluble phenylpropanoid accumulation, reduced lignin content and dwarfism. By contrast, loss of <jats:styled-content style=\"fixed-case\">MED<\/jats:styled-content>5 results in increased concentrations of phenylpropanoids.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>We used a reverse genetic approach to identify suppressors of <jats:italic>ref4\u20103<\/jats:italic> and found that <jats:italic>ref4\u20103<\/jats:italic> requires <jats:styled-content style=\"fixed-case\">CDK<\/jats:styled-content>8, a kinase module subunit of Mediator, to repress plant growth. The genetic interaction between <jats:italic><jats:styled-content style=\"fixed-case\">MED<\/jats:styled-content>5<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">CDK<\/jats:styled-content>8<\/jats:italic> was further characterized using m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010sequencing (<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010seq) and metabolite analysis.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Growth inhibition and suppression of phenylpropanoid metabolism can be genetically separated in <jats:italic>ref4\u20103<\/jats:italic> by elimination of <jats:styled-content style=\"fixed-case\">CDK<\/jats:styled-content>8 kinase activity; however, the stunted growth of <jats:italic>ref4\u20103<\/jats:italic> is not dependent on the phosphorylation event introduced by the G383S mutation. In addition, rather than perturbation of lignin biosynthesis, misregulation of <jats:italic><jats:styled-content style=\"fixed-case\">DJC<\/jats:styled-content>66<\/jats:italic>, a gene encoding a <jats:styled-content style=\"fixed-case\">DNAJ<\/jats:styled-content> protein, is involved in the dwarfism of the <jats:italic>med5<\/jats:italic> mutants.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Together, our study reveals genetic interactions between Mediator tail and kinase module subunits and enhances our understanding of dwarfing in phenylpropanoid pathway mutants.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.15741","type":"journal-article","created":{"date-parts":[[2019,2,13]],"date-time":"2019-02-13T09:30:58Z","timestamp":1550050258000},"page":"233-245","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Mutation of Mediator subunit <i><scp>CDK<\/scp>8<\/i> counteracts the stunted growth and salicylic acid hyperaccumulation phenotypes of an Arabidopsis <i><scp>MED<\/scp>5<\/i> mutant"],"prefix":"10.1111","volume":"223","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2748-313X","authenticated-orcid":false,"given":"Xiangying","family":"Mao","sequence":"first","affiliation":[{"name":"Department of Biochemistry Purdue University West Lafayette IN 47907 USA"},{"name":"Purdue Center for Plant Biology Purdue University West Lafayette IN 47907 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5618-3948","authenticated-orcid":false,"given":"Jeong Im","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Purdue University West Lafayette IN 47907 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitchell T.","family":"Wheeler","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Purdue University West Lafayette IN 47907 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne K.","family":"Heintzelman","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Purdue University West Lafayette IN 47907 USA"},{"name":"Northwest Missouri State University Maryville MO 64468 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5933-9952","authenticated-orcid":false,"given":"Vikki M.","family":"Weake","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Purdue University West Lafayette IN 47907 USA"},{"name":"Purdue University Center for Cancer Research Purdue University West Lafayette IN 47907 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5195-562X","authenticated-orcid":false,"given":"Clint","family":"Chapple","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Purdue University West Lafayette IN 47907 USA"},{"name":"Purdue Center for Plant Biology Purdue University West Lafayette IN 47907 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu638"},{"key":"e_1_2_7_3_1","first-page":"1557","article-title":"Loss of ferulate 5\u2010hydroxylase leads to Mediator\u2010dependent inhibition of soluble phenylpropanoid biosynthesis in Arabidopsis","volume":"169","author":"Anderson N","year":"2015","journal-title":"Plant Physiology"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1203877"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5404.985"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13084"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.312298"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M207195200"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81858-9"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0070384"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.1998.00343.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2011.01.013"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1199\/tab.0156"},{"key":"e_1_2_7_14_1","first-page":"4","article-title":"Conservation and divergence of Mediator structure and function: insights from plants","volume":"58","author":"Dolan WL","year":"2017","journal-title":"Plant Cell Physiology"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.17.00282"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.1.207"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/07352689.2015.1078613"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1117873108"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2010.03621.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1307525111"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400221101"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80112-4"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.211"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/mp\/ssu049"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1600739113"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf348"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2009.04.005"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.15.00127"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.104935"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1767009"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-017-0687-4"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.9.4006"},{"key":"e_1_2_7_33_1","first-page":"2409","article-title":"Four isoforms of Arabidopsis thaliana 4\u2010coumarate: CoA ligase (4CL) have overlapping yet distinct roles in phenylpropanoid metabolism","volume":"169","author":"Li Y","year":"2015","journal-title":"Plant Physiology"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2901"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks042"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2013.10.004"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.416727"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature22328"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.03.030"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202399"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-005-3326-x"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/159.4.1741"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2015.05.011"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090322"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.2003.01890.x"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.107.083881"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2020"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2549"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.05.015"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature21393"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01187"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.2307\/3869297"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.12377"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.11.13.1621"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.114.128611"}],"container-title":["New Phytologist"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnph.15741","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nph.15741","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/nph.15741","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/am-pdf\/10.1111\/nph.15741","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nph.15741","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T10:22:14Z","timestamp":1692526934000},"score":1,"resource":{"primary":{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/10.1111\/nph.15741"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,18]]},"references-count":54,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["10.1111\/nph.15741"],"URL":"https:\/\/doi.org\/10.1111\/nph.15741","archive":["Portico"],"relation":{},"ISSN":["0028-646X","1469-8137"],"issn-type":[{"value":"0028-646X","type":"print"},{"value":"1469-8137","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,18]]},"assertion":[{"value":"2019-01-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}