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Previously we showed that plants in which expression of the <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content> protein coilin is downregulated are more susceptible to certain viruses including tobacco rattle virus (<jats:styled-content style=\"fixed-case\">TRV<\/jats:styled-content>), suggesting a role of coilin in antiviral defence.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Experiments with coilin\u2010deficient plants and the deletion mutant of the <jats:styled-content style=\"fixed-case\">TRV<\/jats:styled-content> 16K protein showed that both 16K and coilin are required for restriction of systemic <jats:styled-content style=\"fixed-case\">TRV<\/jats:styled-content> infection. The potential mechanisms of coilin\u2010mediated antiviral defence were elucidated via experiments involving co\u2010immunoprecipitation, use of NahG transgenic plants deficient in salicylic acid (<jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>) accumulation, measurement of endogenous <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> concentrations and assessment of <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>\u2010responsive gene expression.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Here we show that <jats:styled-content style=\"fixed-case\">TRV<\/jats:styled-content> 16K interacts with and relocalizes coilin to the nucleolus. In wild\u2010type plants these events are accompanied by activation of <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>\u2010responsive gene expression and restriction of <jats:styled-content style=\"fixed-case\">TRV<\/jats:styled-content> systemic infection. By contrast, viral systemic spread was enhanced in NahG plants, implicating <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> in these processes.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Our findings suggest that coilin is involved in plant defence, responding to <jats:styled-content style=\"fixed-case\">TRV<\/jats:styled-content> infection by recognition of the <jats:styled-content style=\"fixed-case\">TRV<\/jats:styled-content>\u2010encoded 16K protein and activating <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>\u2010dependent defence pathways.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.15994","type":"journal-article","created":{"date-parts":[[2019,6,19]],"date-time":"2019-06-19T10:04:48Z","timestamp":1560938688000},"page":"439-453","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Interaction of a plant virus protein with the signature Cajal body protein coilin facilitates salicylic acid\u2010mediated plant defence responses"],"prefix":"10.1111","volume":"224","author":[{"given":"Jane","family":"Shaw","sequence":"first","affiliation":[{"name":"Cell and Molecular Sciences The James Hutton Institute Invergowrie Dundee DD2 5DA UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chulang","family":"Yu","sequence":"additional","affiliation":[{"name":"Cell and Molecular Sciences The James Hutton Institute Invergowrie Dundee DD2 5DA UK"},{"name":"State Key Laboratory for Quality and Safety of Agro-products, Institute of Plant Virology Ningbo University Ningbo 117997 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonida V.","family":"Makhotenko","sequence":"additional","affiliation":[{"name":"Shemyakin\u2010Ovchinnikov Institute of Bioorganic Chemistry of the RAS Moscow 117997 Russia"},{"name":"A.N. 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