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Extracellular resistance involves products of\n PEN<\/jats:styled-content>\n 2\u2010dependent metabolism of indole glucosinolates (\n IG<\/jats:styled-content>\n s). Restriction of further fungal growth requires\n PAD<\/jats:styled-content>\n 3\u2010dependent camalexin and other, as yet uncharacterized, indolics.\n <\/jats:p>\n <\/jats:list-item>\n \n \n This study focuses on the function of\n CYP<\/jats:styled-content>\n 71A12 monooxygenase in pathogen\u2010triggered Trp metabolism, including the biosynthesis of indole\u20103\u2010carboxylic acid (\n ICA<\/jats:styled-content>\n ). Moreover, to investigate the contribution of\n CYP<\/jats:styled-content>\n 71A12 and its products to Arabidopsis immunity, we analyzed infection phenotypes of multiple mutant lines combining\n pen2<\/jats:italic>\n with\n pad3<\/jats:italic>\n ,\n cyp71A12<\/jats:italic>\n ,\n cyp71A13<\/jats:italic>\n or\n cyp82C2<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:list-item>\n \n \n Metabolite profiling of\n cyp71A12<\/jats:italic>\n lines revealed a reduction in\n ICA<\/jats:styled-content>\n accumulation. Additionally, analysis of mutant plants showed that low amounts of\n ICA<\/jats:styled-content>\n can form during an immune response by\n CYP<\/jats:styled-content>\n 71B6\/\n AAO<\/jats:styled-content>\n 1\u2010dependent metabolism of indole acetonitrile, but not via\n IG<\/jats:styled-content>\n hydrolysis. Infection assays with\n Plectosphaerella cucumerina<\/jats:italic>\n and\n Colletotrichum tropicale<\/jats:italic>\n , two pathogens with different lifestyles, revealed\n cyp71A12\u2010<\/jats:italic>\n ,\n cyp71A13\u2010<\/jats:italic>\n and\n cyp82C2<\/jats:italic>\n \u2010associated defects associated with Arabidopsis immunity.\n <\/jats:p>\n <\/jats:list-item>\n \n \n Our results indicate that\n CYP<\/jats:styled-content>\n 71A12, but not\n CYP<\/jats:styled-content>\n 71A13, is the major enzyme responsible for the accumulation of\n ICA<\/jats:styled-content>\n in Arabidopsis in response to pathogen ingression. We also show that both enzymes are key players in the resistance of Arabidopsis against selected filamentous pathogens after they invade.\n <\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>","DOI":"10.1111\/nph.16118","type":"journal-article","created":{"date-parts":[[2019,8,14]],"date-time":"2019-08-14T11:27:53Z","timestamp":1565782073000},"page":"400-412","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":90,"title":["The role of\n CYP<\/scp>\n 71A12 monooxygenase in pathogen\u2010triggered tryptophan metabolism and Arabidopsis immunity"],"prefix":"10.1111","volume":"225","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9747-8283","authenticated-orcid":false,"given":"Marta","family":"Pastorczyk","sequence":"first","affiliation":[{"name":"Institute of Bioorganic Chemistry Polish Academy of Sciences Noskowskiego 12\/14 61\u2010704 Pozna\u0144 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(Madrid) Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7067-2721","authenticated-orcid":false,"given":"Henning","family":"Frerigmann","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Plant Breeding Research and Cluster of Excellence on Plant Sciences (CEPLAS) Carl\u2010von\u2010Linn\u00e9\u2010Weg 10 D\u201050829 K\u00f6ln Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina","family":"Ku\u0142ak","sequence":"additional","affiliation":[{"name":"Institute of Bioorganic Chemistry Polish Academy of Sciences Noskowskiego 12\/14 61\u2010704 Pozna\u0144 Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9280-5065","authenticated-orcid":false,"given":"Erich","family":"Glawischnig","sequence":"additional","affiliation":[{"name":"Chair of Botany Department of Plant Sciences Technical University of Munich Emil\u2010Ramann\u2010Str. 4 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