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Microalgae have developed adaptation mechanisms to P limitation that involve remodelling of internal phosphate resources and accumulation of lipids.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Here, we used <jats:italic>in silico<\/jats:italic> analyses to identify the P\u2010stress regulator PtPSR (<jats:italic>Phaeodactylum tricornutum<\/jats:italic> phosphorus starvation response) in the diatom <jats:italic>P. tricornutum<\/jats:italic>. <jats:italic>ptpsr<\/jats:italic> mutant lines were generated using gene editing and characterised by various molecular, genetics and biochemical tools.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>PtPSR belongs to a clade of Myb transcription factors that are conserved in stramenopiles and distantly related to plant P\u2010stress regulators. PtPSR bound specifically to a conserved <jats:italic>cis<\/jats:italic>\u2010regulatory element found in the regulatory region of P\u2010stress\u2010induced genes. <jats:italic>ptpsr<\/jats:italic> knockout mutants showed reduction in cell growth under P limitation. P\u2010stress responses were impaired in <jats:italic>ptpsr<\/jats:italic> mutants compared with wild\u2010type, including reduced induction of P\u2010stress response genes, near to complete loss of alkaline phosphatase activity and reduced phospholipid degradation.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>We conclude that PtPSR is a key transcription factor influencing P scavenging, phospholipid remodelling and cell growth in adaptation to P stress in diatoms.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.16248","type":"journal-article","created":{"date-parts":[[2019,10,10]],"date-time":"2019-10-10T00:33:02Z","timestamp":1570667582000},"page":"2380-2395","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["The Myb\u2010like transcription factor phosphorus starvation response (PtPSR) controls conditional P acquisition and remodelling in marine 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