{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T04:44:54Z","timestamp":1790397894358,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,2,3]],"date-time":"2020-02-03T00:00:00Z","timestamp":1580688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31520103914"],"award-info":[{"award-number":["31520103914"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>\n<jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>How cadmium (Cd) tolerance in rice is regulated remains poorly understood. We used a forward genetic approach to investigate Cd tolerance in rice.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Using a root elongation assay, we isolated a rice mutant with enhanced Cd tolerance, <jats:italic>cadt1<\/jats:italic>, from an ethyl methanesulphonate (EMS)\u2010mutagenized population of a widely grown Indica cultivar. The mutant accumulated more Cd in roots but not in shoots and grains. Using genomic resequencing and complementation, we identified <jats:italic>OsCADT1<\/jats:italic> as the causal gene for the mutant phenotype, which encodes a putative serine hydroxymethyltransferase.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>OsCADT1 protein was localized to the nucleus and the <jats:italic>OsCADT1<\/jats:italic> gene was expressed in both roots and shoots. <jats:italic>OsCADT1<\/jats:italic> mutation resulted in higher sulphur and selenium accumulation in the shoots and grains. Selenate influx in <jats:italic>cadt1<\/jats:italic> was 2.4 times that of the wild\u2010type. The mutant showed higher expression of the sulphate\/selenate transporter gene <jats:italic>OsSULTR1;1<\/jats:italic> and the sulphur\u2010deficiency\u2010inducible gene <jats:italic>OsSDI1<\/jats:italic>. Thiol compounds including cysteine, glutathione and phytochelatins were significantly increased in the mutant, underlying its increased Cd tolerance. Growth and grain biomass were little affected.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The results suggest that OsCADT1 acts as a negative regulator of sulphate\/selenate uptake and assimilation. <jats:italic>OsCADT1<\/jats:italic> mutation increases Cd tolerance and enriches selenium in rice grains, providing a novel solution for selenium biofortification.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.16404","type":"journal-article","created":{"date-parts":[[2019,12,27]],"date-time":"2019-12-27T07:59:58Z","timestamp":1577433598000},"page":"838-850","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":99,"title":["Mutation in <i>OsCADT1<\/i> enhances cadmium tolerance and enriches selenium in rice 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