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Many genes participate in these processes, transcriptionally controlled by Fe\u2010status. Thorough knowledge of the translational check\u2010points is lacking.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>We identified a <jats:italic>non\u2010response to Fe\u2010deficiency1<\/jats:italic>\u2010<jats:italic>1<\/jats:italic> (<jats:italic>nrf1\u20101<\/jats:italic>) mutant of <jats:italic>Arabidopsis thaliana<\/jats:italic>, which displayed a hypersensitive phenotype under Fe\u2010deficient conditions. By mapping <jats:italic>nrf1\u20101<\/jats:italic>, we found that the <jats:italic>AT3G13440<\/jats:italic> locus encoding a HemK methyltransferase is responsible for the phenotype. Analyses of <jats:italic>ProUBQ10:NRF1<jats:sup>CDS<\/jats:sup><\/jats:italic> overexpression <jats:italic>nrf1\u20101<\/jats:italic> lines and a T\u2010DNA insertion mutant <jats:italic>nrf1\u20102<\/jats:italic>, confirmed that loss\u2010of\u2010function of NRF1 results in enhanced Fe\u2010starvation\u2010sensitivity.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>NRF1 is required for the proper expression of the majority of Fe\u2010deficiency\u2010inducible (FDI) genes. The <jats:italic>nrf1<\/jats:italic> mutants accumulated more polysomes in the roots, due to stalled ribosomes on several transcripts. Ribosome\u2010footprint (RF) mapping revealed that ribosomes are stalled at a stop codon that amplified the stalling of trailing ribosomes. We detected higher RF levels in many FDI transcripts in <jats:italic>nrf1\u20102<\/jats:italic>.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Our study demonstrates the requirement of NRF1 for an accurate termination of protein synthesis essential not only for a precise iron homeostasis, but also cellular ion balance. NRF1 is also important for normal growth and development. A check\u2010point that fine\u2010tunes peptide release in plants is uncovered.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.16440","type":"journal-article","created":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T18:36:51Z","timestamp":1579718211000},"page":"1361-1374","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["A HemK class glutamine\u2010methyltransferase is involved in the termination of translation and essential for iron homeostasis in Arabidopsis"],"prefix":"10.1111","volume":"226","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6708-4363","authenticated-orcid":false,"given":"Sakthivel","family":"Kailasam","sequence":"first","affiliation":[{"name":"Agricultural Biotechnology Research Center Academia Sinica Taipei 11529 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