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It is necessary to figure out the initiation and development regulatory mechanism of GSTs to cultivate high\u2010yielding <jats:italic>Artemisia annua<\/jats:italic>.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Here, we identified an MYB transcription factor, AaTAR2, from bioinformatics analysis of the <jats:italic>A<\/jats:italic>.<jats:italic>\u00a0annua<\/jats:italic> genome database and <jats:italic>Arabidopsis<\/jats:italic> trichome development\u2010related genes. AaTAR2 is mainly expressed in young leaves and located in the nucleus. Repression and overexpression of AaTAR2 resulted in a decrease and increase, respectively, in the GSTs numbers, leaf biomass, and the artemisinin content in transgenic plants. Furthermore, the morphological characteristics changed obviously in trichomes, suggesting AaTAR2 plays a key role in trichome formation.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>In addition, the expression of flavonoid biosynthesis genes and total flavonoid content increased dramatically in <jats:italic>AaTAR2<\/jats:italic>\u2010overexpressing transgenic plants. Owing to flavonoids possibly counteracting emerging resistance to artemisinin in <jats:italic>Plasmodium<\/jats:italic> species, AaTAR2 is a potential target to improve the effect of artemisinin in clinical therapy.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Taken together, AaTAR2 positively regulates trichome development and artemisinin and flavonoid biosynthesis. A better understanding of this \u2018multiple functions\u2019 transcription factor may enable enhanced artemisinin and flavonoids yield. AaTAR2 is a potential breeding target for cultivating high\u2010quality <jats:italic>A<\/jats:italic>.<jats:italic>\u00a0annua<\/jats:italic>.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.16777","type":"journal-article","created":{"date-parts":[[2020,6,27]],"date-time":"2020-06-27T09:41:01Z","timestamp":1593250861000},"page":"932-945","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":77,"title":["<i>TRICHOME AND ARTEMISININ REGULATOR 2<\/i> positively regulates trichome development and artemisinin biosynthesis in <i>Artemisia annua<\/i>"],"prefix":"10.1111","volume":"228","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3433-751X","authenticated-orcid":false,"given":"Zheng","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Botany School of Pharmacy Second Military Medical University Shanghai, 200433 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