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Sites of GA biosynthesis in the roots of 7\u2010d\u2010old\n                          <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                          seedlings were investigated using tissue\u2010specific GA inactivation in wild\u2010type (Col\u20100) or rescue of GA\u2010deficient dwarf mutants.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Tissue\u2010specific GA depletion was achieved by ectopic expression of the GA\u2010inactivating enzyme AtGA2ox2, which is specific for C\n                          <jats:sub>19<\/jats:sub>\n                          \u2010GAs, and AtGA2ox7, which acts on C\n                          <jats:sub>20<\/jats:sub>\n                          \u2010GA precursors. In addition, tissue\u2010specific rescue of\n                          <jats:italic>ga20ox<\/jats:italic>\n                          triple and\n                          <jats:italic>ga3ox<\/jats:italic>\n                          double mutants was shown. Furthermore, GUS reporter lines for major\n                          <jats:italic>GA20ox, GA3ox<\/jats:italic>\n                          and\n                          <jats:italic>GA2ox<\/jats:italic>\n                          genes were used to observe their expression domains in the root.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>The effects of expressing these constructs on the lengths of the root apical meristem and cortical cells in the elongation zone confirmed that roots are autonomous for GA biosynthesis, which occurs in multiple tissues, with the endodermis a major site of synthesis.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>The results are consistent with the early stages of GA biosynthesis within the root occurring in the meristematic region and indicate that the penultimate step of GA biosynthesis, GA 20\u2010oxidation, is required in both the meristem and elongation zone.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.16967","type":"journal-article","created":{"date-parts":[[2020,9,29]],"date-time":"2020-09-29T03:35:56Z","timestamp":1601350556000},"page":"1521-1534","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":73,"title":["Mapping sites of gibberellin biosynthesis in the Arabidopsis root tip"],"prefix":"10.1111","volume":"229","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9951-1382","authenticated-orcid":false,"given":"Richard","family":"Barker","sequence":"first","affiliation":[{"name":"Rothamsted Research Harpenden, Hertfordshire, AL5 2JQ UK"},{"name":"Plant &amp; Crop Sciences School of Biosciences University of Nottingham Sutton Bonington, LE12 5RD 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