{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T18:56:55Z","timestamp":1791572215609,"version":"4.3.4"},"reference-count":69,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T00:00:00Z","timestamp":1642723200000},"content-version":"am","delay-in-days":345,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2021,2,10]],"date-time":"2021-02-10T00:00:00Z","timestamp":1612915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IOS\u20101740560"],"award-info":[{"award-number":["IOS\u20101740560"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IOS\u20101856741"],"award-info":[{"award-number":["IOS\u20101856741"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP160102888"],"award-info":[{"award-number":["DP160102888"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["FT150100162"],"award-info":[{"award-number":["FT150100162"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>Strigolactones and karrikins are butenolide molecules that regulate plant growth. They are perceived by the \u03b1\/\u03b2\u2010hydrolase DWARF14 (D14) and its homologue KARRIKIN INSENSITIVE2 (KAI2), respectively. Plant\u2010derived strigolactones have a butenolide ring with a methyl group that is essential for bioactivity. By contrast, karrikins are abiotic in origin, and the butenolide methyl group is nonessential. KAI2 is probably a receptor for an endogenous butenolide, but the identity of this compound remains unknown.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Here we characterise the specificity of KAI2 towards differing butenolide ligands using genetic and biochemical approaches.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          We find that KAI2 proteins from multiple species are most sensitive to desmethyl butenolides that lack a methyl group. Desmethyl\u2010GR24 and desmethyl\u2010CN\u2010debranone are active by KAI2 but not D14. They are more potent KAI2 agonists compared with their methyl\u2010substituted reference compounds both\n                          <jats:italic>in vitro<\/jats:italic>\n                          and in plants. The preference of KAI2 for desmethyl butenolides is conserved in\n                          <jats:italic>Selaginella moellendorffii<\/jats:italic>\n                          and\n                          <jats:italic>Marchantia polymorpha<\/jats:italic>\n                          , suggesting that it is an ancient trait in land plant evolution.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Our findings provide insight into the mechanistic basis for differential ligand perception by KAI2 and D14, and support the view that the endogenous substrates for KAI2 and D14 have distinct chemical structures and biosynthetic origins.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.17224","type":"journal-article","created":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T04:21:21Z","timestamp":1611202881000},"page":"1003-1016","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Desmethyl butenolides are optimal ligands for karrikin receptor proteins"],"prefix":"10.1111","volume":"230","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2945-4746","authenticated-orcid":false,"given":"Jiaren","family":"Yao","sequence":"first","affiliation":[{"name":"School of Molecular Sciences The University of Western Australia Perth WA 6009 Australia"},{"name":"ARC Centre of Excellence in Plant Energy Biology The University of Western Australia Perth WA 6009 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Scaffidi","sequence":"additional","affiliation":[{"name":"School of Molecular Sciences The University of Western Australia Perth WA 6009 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3806-0581","authenticated-orcid":false,"given":"Yongjie","family":"Meng","sequence":"additional","affiliation":[{"name":"School of Molecular Sciences The University of Western Australia Perth WA 6009 Australia"},{"name":"ARC Centre of Excellence in Plant Energy Biology The University of Western Australia Perth WA 6009 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3164-2810","authenticated-orcid":false,"given":"Kim T.","family":"Melville","sequence":"additional","affiliation":[{"name":"School of Molecular Sciences The University of Western Australia Perth WA 6009 Australia"},{"name":"ARC Centre of Excellence in Plant Energy Biology The University of Western Australia Perth WA 6009 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7511-7236","authenticated-orcid":false,"given":"Aino","family":"Komatsu","sequence":"additional","affiliation":[{"name":"Graduate School of Life Sciences Tohoku University Sendai 980\u20108577 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8533-4269","authenticated-orcid":false,"given":"Aashima","family":"Khosla","sequence":"additional","affiliation":[{"name":"Department of Botany and Plant Sciences University of California Riverside CA 92521 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9792-5015","authenticated-orcid":false,"given":"David C.","family":"Nelson","sequence":"additional","affiliation":[{"name":"Department of Botany and Plant Sciences University of California Riverside CA 92521 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junko","family":"Kyozuka","sequence":"additional","affiliation":[{"name":"Graduate School of Life Sciences Tohoku University Sendai 980\u20108577 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2545-6939","authenticated-orcid":false,"given":"Gavin R.","family":"Flematti","sequence":"additional","affiliation":[{"name":"School of Molecular Sciences The University of Western Australia Perth WA 6009 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5657-9005","authenticated-orcid":false,"given":"Mark T.","family":"Waters","sequence":"additional","affiliation":[{"name":"School of Molecular Sciences The University of Western Australia Perth WA 6009 Australia"},{"name":"ARC Centre of Excellence in Plant Energy Biology The University of Western Australia Perth WA 6009 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1410801111"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03608"},{"key":"e_1_2_7_4_1","first-page":"1","article-title":"Strigolactones, a novel carotenoid\u2010derived plant hormone","volume":"66","author":"Al\u2010Babili S","year":"2014","journal-title":"Annual Review of Plant Biology"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1218094"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0040226"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2012.10.001"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.195826"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-020-0653-z"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/pld3.263"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2019.12.009"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.01.003"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-017-0397-z"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2006111117"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.0c00192"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16021-1"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aab1140"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2015.01219"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2012.04209.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1099944"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0633241"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1356"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2019.07.018"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms151222518"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(02)50957-5"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1306265110"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aac9715"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2012.08.007"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0099206"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.16938"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12870"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.19.00752"},{"key":"e_1_2_7_33_1","first-page":"1581","article-title":"SMAX1\u2010LIKE7 signals from the nucleus to regulate shoot development in Arabidopsis via partially EAR motif\u2010independent mechanisms","volume":"28","author":"Liang Y","year":"2016","journal-title":"The Plant Cell"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2019.05.001"},{"key":"e_1_2_7_35_1","doi-asserted-by":"crossref","unstructured":"MizunoY KomatsuA ShimazakiS XieX IshizakiK NaramotoS KyozukaJ.2020.Major components in the KARRIKIN INSENSITIVE2\u2010ligand signaling pathway are conserved in the liverwort Marchantia polymorpha. bioRxiv: 2020.2011.2017.387852.","DOI":"10.1101\/2020.11.17.387852"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911635107"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-042811-105545"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1100987108"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2147"},{"key":"e_1_2_7_40_1","doi-asserted-by":"crossref","unstructured":"deSaint GermainA JacobsA BrunG PouvreauJ\u2010B BraemL CornuD Clav\u00e9G BauduE SteinmetzV ServajeanVet al.2020.APhelipanche ramosaKAI2 protein perceives enzymatically strigolactones and isothiocyanates.bioRxiv: 10.1101\/2020.06.09.136473.","DOI":"10.1101\/2020.06.09.136473"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2012.04.016"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.12265"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.114.240036"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-08124-7"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0743-5"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.15.00562"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-015-2458-2"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.113.221259"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-14991-w"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.14233"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00572-020-00965-9"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2014.07.005"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aab3831"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau5445"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.01641"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008327"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-019-0689-6"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.15.00605"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.20.00140"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-042916-040925"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.074567"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2014.12.020"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.15.00146"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.14017"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erz548"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature19073"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ery090"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2015.122"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.20.00123"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12878"}],"container-title":["New Phytologist"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nph.17224","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/nph.17224","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/am-pdf\/10.1111\/nph.17224","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nph.17224","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T02:37:40Z","timestamp":1693535860000},"score":1,"resource":{"primary":{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/10.1111\/nph.17224"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,10]]},"references-count":69,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,5]]}},"alternative-id":["10.1111\/nph.17224"],"URL":"https:\/\/doi.org\/10.1111\/nph.17224","archive":["Portico"],"relation":{},"ISSN":["0028-646X","1469-8137"],"issn-type":[{"value":"0028-646X","type":"print"},{"value":"1469-8137","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,10]]},"assertion":[{"value":"2020-11-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}