{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T12:18:03Z","timestamp":1788956283228,"version":"build-2803163510"},"reference-count":77,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,4,12]],"date-time":"2021-04-12T00:00:00Z","timestamp":1618185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["Shanghai Sail Program (19YF1459300)"],"award-info":[{"award-number":["Shanghai Sail Program (19YF1459300)"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>\n<jats:list list-type=\"bullet\">\n\n<jats:list-item><jats:p>Plants synthesize diverse diterpenoids with numerous functions in organ development and stress resistance. However, the role of diterpenoids in glandular trichome (GT) development and GT\u2010localized biosynthesis in plants remains unknown.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Here, the identification of 10 diterpene synthases (diTPSs) revealed the diversity of diterpenoid biosynthesis in <jats:italic>Artemisia<\/jats:italic> <jats:italic>annua<\/jats:italic>. Protein\u2013protein interactions (PPIs) between <jats:italic>Aa<\/jats:italic>KSL1 and <jats:italic>Aa<\/jats:italic>CPS2 in the plastids highlighted their potential functions in modulating metabolic flux to gibberellins (GAs) or <jats:italic>ent\u2010<\/jats:italic>isopimara\u20107,15\u2010diene\u2010derived metabolites (IDMs) through metabolic engineering.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>A phenotypic analysis of transgenic plants suggested a complex repertoire of diterpenoids in <jats:italic>Artemisia annua<\/jats:italic> with important roles in GT formation, artemisinin accumulation and stress resilience. Metabolic engineering of diterpenoids simultaneously increased the artemisinin yield and stress resistance. Transcriptome and metabolic profiling suggested that bioactive GA<jats:sub>4<\/jats:sub>\/GA<jats:sub>1<\/jats:sub> promote GT formation.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Collectively, these results expand our knowledge of diterpenoids and show the potential of diterpenoids to simultaneously improve both the GT\u2010localized metabolite yield and stress resistance, <jats:italic>in<\/jats:italic> <jats:italic>planta<\/jats:italic>.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.17351","type":"journal-article","created":{"date-parts":[[2021,3,19]],"date-time":"2021-03-19T16:11:25Z","timestamp":1616170285000},"page":"2387-2403","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Discovery and modulation of diterpenoid metabolism improves glandular trichome formation, artemisinin production and stress resilience in <i>Artemisia annua<\/i>"],"prefix":"10.1111","volume":"230","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7933-3670","authenticated-orcid":false,"given":"Ruibing","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Botany School of Pharmacy Naval Medical University Shanghai 200433 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuejuan","family":"Bu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany School of Pharmacy Naval Medical University Shanghai 200433 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junze","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany School of Pharmacy Naval Medical University Shanghai 200433 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyle A.","family":"Pelot","sequence":"additional","affiliation":[{"name":"Department of Plant Biology University of California Davis CA 95616 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