{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T17:43:05Z","timestamp":1790530985262,"version":"4.1.0"},"reference-count":78,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T00:00:00Z","timestamp":1653955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/H000283\/1"],"award-info":[{"award-number":["BB\/H000283\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000324","name":"Gatsby Charitable Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000324","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>Extracellular ATP is a purinergic signal with important functions in regulating plant growth and stress\u2010adaptive responses, including programmed cell death. While signalling events proximate to receptor activation at the plasma membrane have been characterised, downstream protein targets and the mechanism of cell death activation\/regulation are unknown.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>We designed a proteomic screen to identify ATP\u2010responsive proteins in Arabidopsis cell cultures exposed to mycotoxin stress via fumonisin B1 (FB1) application.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Arabidopsis RIBONUCLEASE 1 (RNS1) was identified by the screen, and transgenic plants overexpressing native RNS1 showed greater susceptibility to FB1, while a gene knockout\n                          <jats:italic>rns1<\/jats:italic>\n                          mutant and antisense\n                          <jats:italic>RNS1<\/jats:italic>\n                          transgenic plants were resistant to FB1\u2010induced cell death. Native RNS1 complemented\n                          <jats:italic>rns1<\/jats:italic>\n                          mutants and restored the cell death response to FB1, while a catalytically inactive version of the ribonuclease could not. The FB1 resistance of salicylic acid (SA)\u2010depleted\n                          <jats:italic>nahG<\/jats:italic>\n                          \u2010expressing plants was abolished by transformation with native RNS1, but not the catalytically dead version.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          The mechanism of FB1\u2010induced cell death is activation of RNS1\u2010dependent RNA cleavage, which is blocked by ATP via\n                          <jats:italic>RNS1<\/jats:italic>\n                          suppression, or enhanced by SA through induction of\n                          <jats:italic>RNS1<\/jats:italic>\n                          expression. Our study reveals RNS1 as a previously unknown convergence point of ATP and SA signalling in the regulation of stress\u2010induced cell death.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.18211","type":"journal-article","created":{"date-parts":[[2022,5,7]],"date-time":"2022-05-07T02:35:57Z","timestamp":1651890957000},"page":"1531-1542","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Extracellular ATP targets Arabidopsis RIBONUCLEASE 1 to suppress mycotoxin stress\u2010induced cell death"],"prefix":"10.1111","volume":"235","author":[{"given":"Heather L.","family":"Goodman","sequence":"first","affiliation":[{"name":"Department of Biosciences Durham University  South Road Durham DH1 3LE UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan T. 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