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It was hypothesized that both polyketide reductase (PKR) and stilbenecarboxylate synthase 1 (STCS1) were involved in the LA biosynthesis, but the underlined mechanisms have not been clarified.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>This study used bioinformatics analysis with molecular, biochemical and physiological approaches to characterize STCS1s and PKRs involved in the biosynthesis of LA.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The results indicated that MpSTCS1s from <jats:italic>Marchantia polymorpha<\/jats:italic> catalyzed both C2\u2192C7 aldol\u2010type and C6\u2192C1 Claisen\u2010type cyclization using dihydro\u2010<jats:italic>p<\/jats:italic>\u2010coumaroyl\u2010coenzyme A (CoA) and malonyl\u2010CoA as substrates to yield a C6\u2010C2\u2010C6 skeleton of dihydro\u2010resveratrol following decarboxylation and the C6\u2010C3\u2010C6 type of phloretin <jats:italic>in\u2009vitro<\/jats:italic>. The protein\u2013protein interaction of PKRs with STCS1 (PPI\u2010PS) was revealed and proved essential for LA accumulation when transiently co\u2010expressed in <jats:italic>Nicotiana benthamiana<\/jats:italic>. Moreover, replacement of the active domain of STCS1 with an 18\u2010amino\u2010acid fragment from the chalcone synthase led to the PPI\u2010PS greatly decreasing and diminishing the formation of LA. The replacement also increased the chalcone formation in STCS1s.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Our results highlight a previously unrecognized PPI <jats:italic>in planta<\/jats:italic> that is indispensable for the formation of LA.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p>","DOI":"10.1111\/nph.18408","type":"journal-article","created":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T06:00:34Z","timestamp":1659074434000},"page":"515-531","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Interaction of <scp>PKR<\/scp> with <scp>STCS1<\/scp>: an indispensable step in the biosynthesis of lunularic acid in <i>Marchantia polymorpha<\/i>"],"prefix":"10.1111","volume":"237","author":[{"given":"Ting\u2010Ting","family":"Zhu","sequence":"first","affiliation":[{"name":"Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine 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