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However, many species are recalcitrant to somatic embryo formation for unknown reasons, which poses a significant challenge to agriculture, where somatic embryogenesis is an important tool to propagate desired genotypes. The micro\u2010RNA394 (miR394) promotes shoot meristem maintenance in <jats:italic>Arabidopsis thaliana<\/jats:italic>, but the underlying mechanisms have remained elusive.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We analyzed whether miR394 affects indirect somatic embryogenesis and determined the transcriptome of embryogenic callus upon miR394\u2010enhanced somatic embryogenesis.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We show that ectopic miR394 expression enhances somatic embryogenesis in the recalcitrant L<jats:italic>er<\/jats:italic> accession when co\u2010expressed with the transcription factor WUSCHEL (WUS) and that miR394 acts in this process through silencing the target LEAF CURLING RESPONSIVENESS (LCR). Furthermore, we show that higher endogenous miR394 levels are required for the elevated embryogenic potential of the Columbia accession compared with L<jats:italic>er<\/jats:italic>, providing a mechanistic explanation for this natural variation.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Our transcriptional analysis provides a framework for miR394 function in regulating pluripotency by expanding WUS\u2010mediated direct transcriptional repression.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p>","DOI":"10.1111\/nph.18801","type":"journal-article","created":{"date-parts":[[2023,2,8]],"date-time":"2023-02-08T08:18:52Z","timestamp":1675844332000},"page":"1059-1072","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["<scp>miR394<\/scp> enhances <scp>WUSCHEL<\/scp>\u2010induced somatic embryogenesis in <i>Arabidopsis 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