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However, little is known about sulfide\u2010mediated regulation in relation to photorespiration.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Here, we performed label\u2010free quantitative proteomic analysis and observed a high impact on protein persulfidation levels when plants grown under nonphotorespiratory conditions were transferred to air, with 98.7% of the identified proteins being more persulfidated under suppressed photorespiration. Interestingly, a higher level of reactive oxygen species (ROS) was detected under nonphotorespiratory conditions.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Analysis of the effect of sulfide on aspects associated with non\u2010 or photorespiratory growth conditions has demonstrated that it protects plants grown under suppressed photorespiration. Thus, sulfide amends the imbalance of carbon\/nitrogen and restores ATP levels to concentrations like those of air\u2010grown plants; balances the high level of ROS in plants under nonphotorespiratory conditions to reach a cellular redox state similar to that in air\u2010grown plants; and regulates stomatal closure, to decrease the high guard cell ROS levels and induce stomatal aperture. In this way, sulfide signals the CO<jats:sub>2<\/jats:sub>\u2010dependent stomata movement, in the opposite direction of the established abscisic acid\u2010dependent movement.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Our findings suggest that the high persulfidation level under suppressed photorespiration reveals an essential role of sulfide signaling under these conditions.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.18838","type":"journal-article","created":{"date-parts":[[2023,2,25]],"date-time":"2023-02-25T07:44:36Z","timestamp":1677311076000},"page":"1431-1445","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Persulfidation protects from oxidative stress under nonphotorespiratory conditions in Arabidopsis"],"prefix":"10.1111","volume":"238","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3595-3963","authenticated-orcid":false,"given":"Margarita","family":"Garc\u00eda\u2010Calder\u00f3n","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica Vegetal y Biolog\u00eda Molecular Universidad de Sevilla  Prof. Garc\u00eda Gonz\u00e1lez 1 41012 Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3377-3187","authenticated-orcid":false,"given":"Thibaut","family":"Vignane","sequence":"additional","affiliation":[{"name":"Leibniz Institute for Analytical Sciences, ISAS e.V.  44227 Dortmund Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0060-0041","authenticated-orcid":false,"given":"Milos R.","family":"Filipovic","sequence":"additional","affiliation":[{"name":"Leibniz Institute for Analytical Sciences, ISAS e.V.  44227 Dortmund Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5132-9824","authenticated-orcid":false,"given":"M. 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