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Using H<jats:sub>2<\/jats:sub>S\u2010 and O<jats:sub>2<\/jats:sub>\u2010sensitive microsensors, we measured the apparent permeance to H<jats:sub>2<\/jats:sub>S of rice roots, tested whether restricted H<jats:sub>2<\/jats:sub>S intrusion reduced its adverse effects on root respiration, and whether H<jats:sub>2<\/jats:sub>S could induce the formation of a ROL barrier.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The ROL barrier reduced apparent permeance to H<jats:sub>2<\/jats:sub>S by almost 99%, greatly restricting H<jats:sub>2<\/jats:sub>S intrusion. The ROL barrier acted as a shield towards H<jats:sub>2<\/jats:sub>S; O<jats:sub>2<\/jats:sub> consumption in roots with a ROL barrier remained unaffected at high H<jats:sub>2<\/jats:sub>S concentration (500\u2009\u03bcM), compared to a 67% decline in roots without a barrier. Importantly, low H<jats:sub>2<\/jats:sub>S concentrations induced the formation of a ROL barrier.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In conclusion, the ROL barrier plays a key role in protecting against H<jats:sub>2<\/jats:sub>S intrusion, and H<jats:sub>2<\/jats:sub>S can act as an environmental signalling molecule for the induction of the barrier. This study demonstrates the multiple functions of the suberized\/lignified outer part of the rice root beyond that of restricting ROL.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p>","DOI":"10.1111\/nph.18883","type":"journal-article","created":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T03:04:15Z","timestamp":1679022255000},"page":"1825-1837","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["The barrier to radial oxygen loss protects roots against hydrogen sulphide intrusion and its toxic effect"],"prefix":"10.1111","volume":"238","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9178-7050","authenticated-orcid":false,"given":"Lucas Le\u00f3n","family":"Peralta Ogorek","sequence":"first","affiliation":[{"name":"The Freshwater Biological Laboratory, Department of Biology University of Copenhagen, Universitetsparken 4  3<sup>rd<\/sup> Floor Copenhagen 2100 Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6182-3236","authenticated-orcid":false,"given":"Hirokazu","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Graduate School of Bioagricultural Sciences Nagoya University  Furo\u2010cho, Chikusa Nagoya 464\u20108601 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7119-2052","authenticated-orcid":false,"given":"Mikio","family":"Nakazono","sequence":"additional","affiliation":[{"name":"Graduate School of Bioagricultural Sciences Nagoya University  Furo\u2010cho, Chikusa Nagoya 464\u20108601 Japan"},{"name":"School of Agriculture and Environment The University of Western Australia  Crawley WA 6009 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0827-946X","authenticated-orcid":false,"given":"Ole","family":"Pedersen","sequence":"additional","affiliation":[{"name":"The Freshwater Biological Laboratory, Department of Biology University of Copenhagen, Universitetsparken 4  3<sup>rd<\/sup> Floor Copenhagen 2100 Denmark"},{"name":"School of Agriculture and Environment The University of Western Australia  Crawley WA 6009 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,7]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"AllamA\u2010WI.1971.Soluble sulfides in rice fields and theirin vitroeffects on rice seedlings. 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