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However, current progress of SDR identification and cloning showed following shortcomings: large near\u2010isogenic lines need to be constructed, and a relatively large population is needed; the cost of whole\u2010genome sequencing and assembly is high.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Herein, the X\/Y chromosomes of <jats:italic>Spinacia oleracea<\/jats:italic> L. subsp. <jats:italic>turkestanica<\/jats:italic> were successfully microdissected and assembled using single\u2010chromosome sequencing.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The assembly length of X and Y chromosome is <jats:italic>c.<\/jats:italic> 192.1 and 195.2\u2009Mb, respectively. Three large inversions existed between X and Y chromosome. The SDR size of X and Y chromosome is <jats:italic>c.<\/jats:italic> 13.2 and 24.1\u2009Mb, respectively. MSY region and six male\u2010biased genes were identified. A Y\u2010chromosome\u2010specific marker in SDR was constructed and used to verify the chromosome assembly quality at cytological level via fluorescence <jats:italic>in\u00a0situ<\/jats:italic> hybridization. Meanwhile, it was observed that the SDR located on long arm of Y chromosome and near the centromere.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Overall, a technical system was successfully established for rapid cloning the SDR and it is also applicable to rapid assembly of specific chromosome in other plants. Furthermore, this study laid a foundation for studying the molecular mechanism of sex chromosome evolution in spinach.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p>","DOI":"10.1111\/nph.19176","type":"journal-article","created":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T03:36:29Z","timestamp":1691033789000},"page":"892-903","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A rapid method for assembly of single chromosome and identification of sex determination region based on single\u2010chromosome sequencing"],"prefix":"10.1111","volume":"240","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6973-5345","authenticated-orcid":false,"given":"Ning","family":"Li","sequence":"first","affiliation":[{"name":"College of Life Sciences Henan Normal University  Xinxiang 453007 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