{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:45:22Z","timestamp":1790689522547,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,8,13]],"date-time":"2023-08-13T00:00:00Z","timestamp":1691884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007128","name":"Natural Science Foundation of Shaanxi Province","doi-asserted-by":"publisher","award":["2021JZ\u201014"],"award-info":[{"award-number":["2021JZ\u201014"]}],"id":[{"id":"10.13039\/501100007128","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004739","name":"Youth Innovation Promotion Association of the Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["2020236"],"award-info":[{"award-number":["2020236"]}],"id":[{"id":"10.13039\/501100004739","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>\n                          Glucose\u20106\u2010phosphate dehydrogenases (G6PDs) are essential regulators of cellular redox. Hydrogen sulfide (H\n                          <jats:sub>2<\/jats:sub>\n                          S) is a small gasotransmitter that improves plant adaptation to stress; however, its role in regulating G6PD oligomerization to resist oxidative stress remains unknown in plants.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Persulfidation of cytosolic G6PDs was analyzed by mass spectrometry (MS). The structural change model of AtG6PD6 homooligomer was built by chemical cross\u2010linking coupled with mass spectrometry (CXMS). We isolated\n                          <jats:italic>AtG6PD6<\/jats:italic>\n                          <jats:sup>\n                            <jats:italic>C159A<\/jats:italic>\n                          <\/jats:sup>\n                          and\n                          <jats:italic>SlG6PDC<\/jats:italic>\n                          <jats:sup>\n                            <jats:italic>C155A<\/jats:italic>\n                          <\/jats:sup>\n                          transgenic lines to confirm the\n                          <jats:italic>in\u00a0vivo<\/jats:italic>\n                          function of persulfidated sites with the\n                          <jats:italic>g6pd5<\/jats:italic>\n                          ,\n                          <jats:italic>6<\/jats:italic>\n                          background.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Persulfidation occurs at Arabidopsis G6PD6 Cystine (Cys)159 and tomato G6PDC Cys155, leading to alterations of spatial distance between lysine (K)491\u2010K475 from 42.0\u2009\u00c5 to 10.3\u2009\u00c5 within the G6PD tetramer. The structural alteration occurs in the structural NADP\n                          <jats:sup>+<\/jats:sup>\n                          binding domain, which governs the stability of G6PD homooligomer. Persulfidation enhances G6PD oligomerization, thereby increasing substrate affinity. Under high salt stress, cytosolic G6PDs activity was inhibited due to oxidative modifications. Persulfidation protects these specific sites and prevents oxidative damage.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          In summary, H\n                          <jats:sub>2<\/jats:sub>\n                          S\u2010mediated persulfidation promotes cytosolic G6PD activity by altering homotetrameric structure. The cytosolic G6PD adaptive regulation with two kinds of protein modifications at the atomic and molecular levels is critical for the cellular stress response.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.19188","type":"journal-article","created":{"date-parts":[[2023,8,14]],"date-time":"2023-08-14T02:01:56Z","timestamp":1691978516000},"page":"626-643","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Persulfidation maintains cytosolic G6PDs activity through changing tetrameric structure and competing cysteine sulfur oxidation under salt stress in Arabidopsis and tomato"],"prefix":"10.1111","volume":"240","author":[{"given":"Xiaofeng","family":"Wang","sequence":"first","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfeng","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology Chinese Academy of Sciences  Harbin 150081 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6448-9170","authenticated-orcid":false,"given":"Ying","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuying","family":"Yi","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5969-062X","authenticated-orcid":false,"given":"Honglei","family":"Jia","sequence":"additional","affiliation":[{"name":"School of Environmental Science and Engineering Shaanxi University of Science &amp; Technology  Xi'an Shaanxi 710021 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fali","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haotian","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuyu","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianjinhong","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3061-8998","authenticated-orcid":false,"given":"Jisheng","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.147975"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erx294"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.01369"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2023604118"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(00)00104-0"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R115.695056"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-022-01252-5"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2015.03.016"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2020.01.004"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2021.07.002"},{"key":"e_1_2_9_12_1","first-page":"2017","article-title":"Structural analysis of clinically relevant pathogenic G6PD variants reveals the importance of tetramerization for G6PD activity","volume":"14","author":"Cunningham AD","year":"2017","journal-title":"Matters"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.112.101279"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2014.02.001"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aax8358"},{"key":"e_1_2_9_16_1","first-page":"55","article-title":"Glutamate synthase activities and protein changes in relation to nitrogen nutrition in barley: the dependence on different plastidic glucose\u20106P dehydrogenase isoforms","volume":"409","author":"Esposito S","year":"2004","journal-title":"Journal of Experimental Botany"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.7b00205"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi401700f"},{"key":"e_1_2_9_19_1","first-page":"4251","article-title":"Signaling by hydrogen sulfide and cyanide through post\u2010translational modification","volume":"70","author":"Gotor C","year":"2019","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2022790118"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcy124"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2021.127964"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2018.02.012"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.483594"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envexpbot.2018.06.034"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12040789"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcl006"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2016.04.013"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.2010.01429.x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.18.00838"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2020.09.020"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.909624"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-012-1374-1"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-023-03029-2"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2021.07.016"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2000464"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20130337"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2019.03.023"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1124\/pharmrev.120.000082"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.12638"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0812902106"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.19.00826"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.15488"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.108423"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2004.02293.x"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-042817-040322"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-050718-100005"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2021.03.007"}],"container-title":["New Phytologist"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nph.19188","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,11]],"date-time":"2023-11-11T01:56:24Z","timestamp":1699667784000},"score":1,"resource":{"primary":{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/10.1111\/nph.19188"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,13]]},"references-count":47,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,10]]}},"alternative-id":["10.1111\/nph.19188"],"URL":"https:\/\/doi.org\/10.1111\/nph.19188","archive":["Portico"],"relation":{},"ISSN":["0028-646X","1469-8137"],"issn-type":[{"value":"0028-646X","type":"print"},{"value":"1469-8137","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,13]]},"assertion":[{"value":"2023-04-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}