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Numerous dwarf and grain\u2010shape mutants have been studied to identify genes that can be used to increase crop yield and improve breeding programs.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>In this study, we characterized a dominant mutant, <jats:italic>dwarf and round grain 1<\/jats:italic> (<jats:italic>drg1\u2010D<\/jats:italic>), in bread wheat (<jats:italic>Triticum aestivum<\/jats:italic> L.). <jats:italic>drg1\u2010D<\/jats:italic> plants exhibit multiple phenotypic changes, including dwarfism, round grains, and insensitivity to brassinosteroids (BR). Cell structure observation in <jats:italic>drg1\u2010D<\/jats:italic> mutant plants showed that the reduced organ size is due to irregular cell shape.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Using map\u2010based cloning and verification in transgenic plants, we found that a Glu209Lys substitution in the DRG1 protein is responsible for the irregular cell size and arrangement in the <jats:italic>drg1\u2010D<\/jats:italic> mutant. <jats:italic>DRG1\/TaACT7<\/jats:italic> encodes an actin family protein that is essential for polymerization stability and microfilament (MF) formation. In addition, the BR response and vesicular transport were altered by the abnormal actin cytoskeleton in <jats:italic>drg1\u2010D<\/jats:italic> mutant plants.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Our study demonstrates that DRG1\/TaACT7 plays an important role in wheat cell shape determination by modulating actin organization and intracellular material transport, which could in the longer term provide tools to better understand the polymerization of actin and its assembly into filaments and arrays.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.19246","type":"journal-article","created":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T12:05:25Z","timestamp":1693915525000},"page":"1913-1929","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A <scp>Glu209Lys<\/scp> substitution in <scp>DRG1<\/scp>\/<scp>TaACT7<\/scp>, which disturbs F\u2010actin organization, reduces 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