{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:46:47Z","timestamp":1791236807080,"version":"4.1.0"},"reference-count":68,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T00:00:00Z","timestamp":1705968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>\n<jats:list list-type=\"bullet\">\n\n<jats:list-item><jats:p>Although polyploid plants have lower stomatal density than their diploid counterparts, the molecular mechanisms underlying this difference remain elusive.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Here, we constructed a network based on the triploid poplar transcriptome data and triple\u2010gene mutual interaction algorithm and found that <jats:italic>PpnMYC2<\/jats:italic> was related to stomatal development\u2010related genes <jats:italic>PpnEPF2<\/jats:italic>, <jats:italic>PpnEPFL4<\/jats:italic>, and <jats:italic>PpnEPFL9<\/jats:italic>. The interactions between PpnMYC2 and PagJAZs were experimentally validated. <jats:italic>PpnMYC2<\/jats:italic>\u2010overexpressing poplar and <jats:italic>Arabidopsis thaliana<\/jats:italic> had reduced stomatal density. Poplar overexpressing <jats:italic>PpnMYC2<\/jats:italic> had higher water use efficiency and drought resistance.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>RNA\u2010sequencing data of poplars overexpressing <jats:italic>PpnMYC2<\/jats:italic> showed that <jats:italic>PpnMYC2<\/jats:italic> promotes the expression of stomatal density inhibitors <jats:italic>PagEPF2<\/jats:italic> and <jats:italic>PagEPFL4<\/jats:italic> and inhibits the expression of the stomatal density\u2010positive regulator <jats:italic>PagEPFL9<\/jats:italic>. Yeast one\u2010hybrid system, electrophoretic mobility shift assay, ChIP\u2010qPCR, and dual\u2010luciferase assay were employed to substantiate that <jats:italic>PpnMYC2<\/jats:italic> directly regulated <jats:italic>PagEPF2<\/jats:italic>, <jats:italic>PagEPFL4<\/jats:italic>, and <jats:italic>PagEPFL9<\/jats:italic>. <jats:italic>PpnMYC2<\/jats:italic>, <jats:italic>PpnEPF2<\/jats:italic>, and <jats:italic>PpnEPFL4<\/jats:italic> were significantly upregulated, whereas <jats:italic>PpnEPFL9<\/jats:italic> was downregulated during stomatal formation in triploid poplar.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Our results are of great significance for revealing the regulation mechanism of plant stomatal occurrence and polyploid stomatal density, as well as reducing stomatal density and improving plant water use efficiency by overexpressing <jats:italic>MYC2<\/jats:italic>.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.19531","type":"journal-article","created":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T08:05:55Z","timestamp":1705997155000},"page":"2506-2522","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["<scp>MYC2<\/scp> regulates stomatal density and water use efficiency via targeting <scp>EPF2<\/scp>\/<scp>EPFL4<\/scp>\/<scp>EPFL9<\/scp> in poplar"],"prefix":"10.1111","volume":"241","author":[{"given":"Yufei","family":"Xia","sequence":"first","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Biotechnology Beijing Forestry University  Beijing 100083 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