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While lots of research has been performed with mutants of <jats:italic>Arabidopsis<\/jats:italic>, little to no data are available for mutants of agricultural crop or tree species.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>By employing a combined set of physiological, histochemical, analytical, and transport physiological methods as well as RNA\u2010sequencing, this study investigated the implications of remarkable CRISPR\/Cas9\u2010induced suberization defects in young roots of the economically important gray poplar.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>While barely affecting overall plant development, contrary to literature\u2010based expectations significant root suberin reductions of up to 80\u201395% in four independent mutants were shown to not evidently affect the root hydraulic conductivity during non\u2010stress conditions. In addition, subliminal iron deficiency symptoms and increased translocation of a photosynthesis inhibitor as well as NaCl highlight the involvement of suberin in nutrient transport physiology.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The multifaceted nature of the root hydraulic conductivity does not allow drawing simplified conclusions such as that the suberin amount must always be correlated with the water transport properties of roots. However, the decreased masking of plasma membrane surface area could facilitate the uptake but also leakage of beneficial and harmful solutes.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p>","DOI":"10.1111\/nph.19588","type":"journal-article","created":{"date-parts":[[2024,2,17]],"date-time":"2024-02-17T03:50:58Z","timestamp":1708141858000},"page":"137-153","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Suberin deficiency and its effect on the transport physiology of young poplar roots"],"prefix":"10.1111","volume":"242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6298-5656","authenticated-orcid":false,"given":"Paul","family":"Gr\u00fcnhofer","sequence":"first","affiliation":[{"name":"Department of Ecophysiology, Institute of Cellular and Molecular Botany University of Bonn  Kirschallee 1 53115 Bonn 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