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In this study, we described and characterized autophagy in the newly identified extremophilic green microalga <jats:italic>Chlamydomonas urium<\/jats:italic>, which was isolated from an acidic environment.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>The nuclear genome of <jats:italic>C. urium<\/jats:italic> was sequenced, assembled and annotated in order to identify autophagy\u2010related genes. Transmission electron microscopy, immunoblotting, metabolomic and photosynthetic analyses were performed to investigate autophagy in this extremophilic microalga.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>The analysis of the <jats:italic>C. urium<\/jats:italic> genome revealed the conservation of core autophagy\u2010related genes. We investigated the role of autophagy in <jats:italic>C. urium<\/jats:italic> by blocking autophagic flux with the vacuolar ATPase inhibitor concanamycin A. Our results indicated that inhibition of autophagic flux in this microalga resulted in a pronounced accumulation of triacylglycerols and lipid droplets (LDs). Metabolomic and photosynthetic analyses indicated that <jats:italic>C. urium<\/jats:italic> cells with impaired vacuolar function maintained an active metabolism. Such effects were not observed in the neutrophilic microalga <jats:italic>Chlamydomonas reinhardtii<\/jats:italic>.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Inhibition of autophagic flux in <jats:italic>C. urium<\/jats:italic> uncovered an active recycling of LDs through lipophagy, a selective autophagy pathway for lipid turnover. This study provided the metabolic basis by which extremophilic algae are able to catabolize lipids in the vacuole.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.19811","type":"journal-article","created":{"date-parts":[[2024,5,11]],"date-time":"2024-05-11T03:55:00Z","timestamp":1715399700000},"page":"284-298","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Lipid turnover through lipophagy in the newly identified extremophilic green microalga <i>Chlamydomonas urium<\/i>"],"prefix":"10.1111","volume":"243","author":[{"given":"Mar\u00eda Esther","family":"P\u00e9rez\u2010P\u00e9rez","sequence":"first","affiliation":[{"name":"Instituto de Bioqu\u00edmica Vegetal y Fotos\u00edntesis (CSIC\u2010Universidad de Sevilla)  41092 Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel 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J.","family":"Salas","sequence":"additional","affiliation":[{"name":"Instituto de la Grasa (CSIC)  Ctra Utrera Km1, Ed. 46 41013 Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrique","family":"Mart\u00ednez\u2010Force","sequence":"additional","affiliation":[{"name":"Instituto de la Grasa (CSIC)  Ctra Utrera Km1, Ed. 46 41013 Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio J.","family":"P\u00e9rez\u2010Pulido","sequence":"additional","affiliation":[{"name":"Centro Andaluz de Biolog\u00eda del Desarrollo (CABD, UPO\u2010CSIC\u2010JA), Faculty of Experimental Sciences (Genetics Department) University Pablo de Olavide  41013 Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3514-1025","authenticated-orcid":false,"given":"Jos\u00e9 L.","family":"Crespo","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica Vegetal y Fotos\u00edntesis 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