{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T17:24:37Z","timestamp":1788283477323,"version":"build-2803163510"},"reference-count":69,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T00:00:00Z","timestamp":1726358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32472726"],"award-info":[{"award-number":["32472726"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>\n                          Hydrogen sulfide (H\n                          <jats:sub>2<\/jats:sub>\n                          S) is an endogenous gaseous signaling molecule, which has been shown to play an important role in plant growth and development by coupling with various phytohormones. However, the relationship between H\n                          <jats:sub>2<\/jats:sub>\n                          S and cytokinin (CTK) and the mechanisms by which H\n                          <jats:sub>2<\/jats:sub>\n                          S and CTK affect root growth remain poorly understood.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Endogenous CTK was analyzed by UHPLC\u2010ESI\u2010MS\/MS. Persulfidation of cytokinin oxidase\/dehydrogenases (CKXs) was analyzed by mass spectrometry (MS).\n                          <jats:italic>ckx2\/CKX2<\/jats:italic>\n                          <jats:sup>\n                            <jats:italic>wild\u2010type (WT)<\/jats:italic>\n                          <\/jats:sup>\n                          , OE\u00a0\n                          <jats:italic>CKX2<\/jats:italic>\n                          and\n                          <jats:italic>ckx2\/CKX2<\/jats:italic>\n                          <jats:sup>\n                            <jats:italic>Cys(C)62alanine(A)<\/jats:italic>\n                          <\/jats:sup>\n                          transgenic lines were isolated with the\n                          <jats:italic>ckx2<\/jats:italic>\n                          background.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          H\n                          <jats:sub>2<\/jats:sub>\n                          S is linked to CTK content by CKX2, which regulates root system architecture (RSA). Persulfidation at cysteine (Cys)62 residue of CKX2 enhances CKX2 activity, resulting in reduced CTK content. We utilized\n                          <jats:italic>35S\u2010LCD\/oasa1<\/jats:italic>\n                          transgenic lines to investigate the effect of endogenous H\n                          <jats:sub>2<\/jats:sub>\n                          S on RSA, indicating that H\n                          <jats:sub>2<\/jats:sub>\n                          S reduces the gravitropic set\u2010point angle (GSA), shortens root hairs, and increases the number of lateral roots (LRs). The persulfidation of CKX2\n                          <jats:sup>Cys62<\/jats:sup>\n                          changes the elongation of cells on the upper and lower flanks of LR elongation zone, confirming that Cys62 of CKX2 is the specificity target of H\n                          <jats:sub>2<\/jats:sub>\n                          S to regulate RSA\n                          <jats:italic>in vivo<\/jats:italic>\n                          .\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          In conclusion, this study demonstrated that H\n                          <jats:sub>2<\/jats:sub>\n                          S negatively regulates CTK content and affects RSA by persulfidation of CKX2\n                          <jats:sup>Cys62<\/jats:sup>\n                          in\n                          <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                          .\n                        <\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.20122","type":"journal-article","created":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T23:45:52Z","timestamp":1726443952000},"page":"1377-1390","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Hydrogen sulfide antagonizes cytokinin to change root system architecture through persulfidation of\n                    <scp>CKX2<\/scp>\n                    in Arabidopsis"],"prefix":"10.1111","volume":"244","author":[{"given":"Xiuyu","family":"Wang","sequence":"first","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuixia","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest 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