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Consequently, many algal groups evolved a CO\n                          <jats:sub>2<\/jats:sub>\n                          \u2010concentrating mechanism (CCM). However, its presence in Charophyceae remains controversial.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          To explore this, we analyzed the acclimation of photosynthesis, carbon isotope composition, and gene expression in\n                          <jats:italic>Chara braunii<\/jats:italic>\n                          under varying inorganic carbon (Ci) conditions.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          The photosynthetic activity changed complementarily under low\u2010 or high\u2010Ci levels. Notably, the Ci compensation point of photosynthesis was significantly lower in thalli grown at ambient Ci than in elevated Ci. Correspondingly, the delta\n                          <jats:sup>13<\/jats:sup>\n                          C levels were lower in thalli from high than low Ci. These results indicate that\n                          <jats:italic>C.\u2009braunii<\/jats:italic>\n                          performs a CCM under low Ci, which is suppressed under high Ci. Transcriptomic analyses of algae from different Ci cultivations provided insight into Ci\u2010regulated genes and pointed to the possible association between carbonic anhydrases and aquaporins with the CCM.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Collectively, our results indicate that\n                          <jats:italic>C.\u2009braunii<\/jats:italic>\n                          expresses a CCM allowing efficient use of CO\n                          <jats:sub>2<\/jats:sub>\n                          and bicarbonate under limiting Ci conditions. A tentative scenario is provided summarizing the role of potential players in the CCM.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.70283","type":"journal-article","created":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T05:06:46Z","timestamp":1750136806000},"page":"1218-1233","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Evidence for a\n                    <scp>\n                      CO\n                      <sub>2<\/sub>\n                    <\/scp>\n                    \u2010concentrating mechanism in the model streptophyte green alga\n                    <i>Chara braunii<\/i>"],"prefix":"10.1111","volume":"247","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-8742-9575","authenticated-orcid":false,"given":"Carolin M.","family":"Heise","sequence":"first","affiliation":[{"name":"Plant Physiology Department Faculty of Mathematics and Natural Sciences, University of Rostock  Einsteinstr. 3 18059 Rostock Germany"},{"name":"Aquatic Ecology Department Faculty of Mathematics and Natural Sciences, University of Rostock  Einsteinstr. 3 18059 Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7869-6923","authenticated-orcid":false,"given":"Daniel A.","family":"He\u00df","sequence":"additional","affiliation":[{"name":"Genetics and Experimental Bioinformatics Group Faculty of Biology, University of Freiburg  Sch\u00e4nzlestr. 1 79104 Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8043-183X","authenticated-orcid":false,"given":"Peter","family":"Walke","sequence":"additional","affiliation":[{"name":"Plant Physiology Department Faculty of Mathematics and Natural Sciences, University of Rostock  Einsteinstr. 3 18059 Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5827-9062","authenticated-orcid":false,"given":"Maren","family":"Vo\u00df","sequence":"additional","affiliation":[{"name":"Marine Nitrogen Cycle, Department of Biological Oceanography Leibniz\u2010Institute for Baltic Sea Research Warnem\u00fcnde (IOW)  18119 Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6309-2609","authenticated-orcid":false,"given":"Hendrik","family":"Schubert","sequence":"additional","affiliation":[{"name":"Aquatic Ecology Department Faculty of Mathematics and Natural Sciences, University of Rostock  Einsteinstr. 3 18059 Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5340-3423","authenticated-orcid":false,"given":"Wolfgang R.","family":"Hess","sequence":"additional","affiliation":[{"name":"Genetics and Experimental Bioinformatics Group Faculty of Biology, University of Freiburg  Sch\u00e4nzlestr. 1 79104 Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2059-2061","authenticated-orcid":false,"given":"Martin","family":"Hagemann","sequence":"additional","affiliation":[{"name":"Plant Physiology Department Faculty of Mathematics and Natural Sciences, University of Rostock  Einsteinstr. 3 18059 Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00709-017-1191-z"},{"key":"e_1_2_9_3_1","unstructured":"AndrewsS.2010.FastQC: a quality control tool for high throughput sequence data. 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