{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T20:51:04Z","timestamp":1791838264259,"version":"4.3.4"},"reference-count":73,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>Iron is indispensable for the vast majority of organisms, and iron homeostasis plays a pivotal role in both the physiology and pathogenesis of fungal pathogens. However, the underlying mechanisms by which iron homeostasis modulates fungal pathogenesis remain to be fully elucidated.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          We therefore focused on investigating the functions of mitochondrial iron transporter ClMrs3\/4 in virulence. We conducted targeted gene deletions, expression analyses, biochemistry, and pathogenicity assays, demonstrating that\n                          <jats:italic>ClMrs3\/4<\/jats:italic>\n                          regulates appressorial development via maintenance of cellular iron balance in\n                          <jats:italic>Curvularia lunata<\/jats:italic>\n                          .\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          <jats:italic>ClMrs3\/4<\/jats:italic>\n                          modulates virulence by influencing appressorial development in\n                          <jats:italic>C. lunata<\/jats:italic>\n                          , which is dependent on iron homeostasis. ClMrs3\/4 controls nitric oxide (NO) balance via the nitrate (NO\n                          <jats:sub>3<\/jats:sub>\n                          <jats:sup>\u2212<\/jats:sup>\n                          ) assimilation pathway by modulating cytoplasmic iron levels, a process crucial for turgor pressure accumulation within the appressoria independent of mitochondrial and cytoplasmic Fe\u2013S cluster biosynthesis.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Our findings underscore the conserved role of Mrs3\/4 in iron homeostasis among pathogenic fungi and propose a novel mechanism by which iron homeostasis regulates virulence, particularly through the NO\n                          <jats:sub>3<\/jats:sub>\n                          <jats:sup>\u2212<\/jats:sup>\n                          assimilation pathway mediated by cytoplasmic iron levels to regulate appressorial development.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.70594","type":"journal-article","created":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T06:19:16Z","timestamp":1758867556000},"page":"2428-2443","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mitochondrial iron transporter\n                    <scp>ClMrs3<\/scp>\n                    \/4 regulates iron homeostasis to modulate nitric oxide balance facilitating appressorial development in\n                    <i>Curvularia lunata<\/i>"],"prefix":"10.1111","volume":"248","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6645-2144","authenticated-orcid":false,"given":"Jiaying","family":"Sun","sequence":"first","affiliation":[{"name":"College of Plant Protection Shenyang Agriculture University  Shenyang 110161 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