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Most pyrenoids are traversed by membranes that are thought to supply them with concentrated CO\n                          <jats:sub>2<\/jats:sub>\n                          . Despite the critical nature of these membranes for pyrenoid function, they are poorly understood, with few protein components known in any species.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Here, we identify protein components of the pyrenoid\u2010traversing membranes from the leading model alga,\n                          <jats:italic>Chlamydomonas reinhardtii,<\/jats:italic>\n                          by affinity purification and mass spectrometry of membrane fragments. Our proteome includes previously known proteins as well as novel candidates.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          We further characterize two of the novel pyrenoid\u2010traversing membrane\u2010resident proteins:\u00a0Cre10.g452250, which we name Pyrenoid Membrane Enriched 1 (PME1), and Cre02.g143550, also known as Low\u2010CO\n                          <jats:sub>2<\/jats:sub>\n                          \u2010Induced 16 (LCI16). We confirm the pyrenoid\u2010traversing membrane localization of LCI16 and observe that PME1 and LCI16 physically interact. We find that neither protein is required for normal membrane morphology or growth under CO\n                          <jats:sub>2<\/jats:sub>\n                          \u2010limiting conditions, but that both mutants show a similar proteomic profile to those of established pyrenoid mutants.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Taken together, our study identifies the proteome of the pyrenoid\u2010traversing membranes and initiates the characterization of a novel pyrenoid\u2010traversing membrane complex, building toward a mechanistic understanding of the pyrenoid.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  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