{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T17:44:16Z","timestamp":1782236656042,"version":"3.54.5"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T00:00:00Z","timestamp":1766102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T00:00:00Z","timestamp":1766102400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173918"],"award-info":[{"award-number":["82173918"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>\n                          Tanshinones, the primary bioactive components found in\n                          <jats:italic>Salvia miltiorrhiza<\/jats:italic>\n                          , are widely utilized in the treatment of cardiovascular diseases; however, the molecular mechanisms that govern their biosynthesis remain unclear at the protein phosphorylation level.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          The proteomic profiles of roots, stems, leaves, and flowers of\n                          <jats:italic>S. miltiorrhiza<\/jats:italic>\n                          identified a total of 8301 proteins. A novel MITOGEN ACTIVATED PROTEIN KINASE 4 (SmMAPK4) was identified to be positively correlated with the accumulation of tanshinones in\n                          <jats:italic>S. miltiorrhiza<\/jats:italic>\n                          through tissue\u2010specific metabolomics and proteomics analyses. Meanwhile, through phosphoproteomic analysis of\n                          <jats:italic>SmMAPK4<\/jats:italic>\n                          overexpression lines, the BASIC HELIX\u2010LOOP\u2010HELIX family transcription factor, BASIC HELIX\u2010LOOP\u2010HELIX TANSHINONE SYNTHESIS 1 (SmBTS1), was screened. The positive regulatory role of SmBTS1 in tanshinone biosynthesis was confirmed through transgenic assays.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Multiple lines of evidence demonstrated that SmMAPK4 could interact with and phosphorylate SmBTS1 in a serine 156\u2010 and serine 159\u2010dependent manner. SmMAPK4\u2010phosphorylated SmBTS1 exhibited enhanced nuclear accumulation and increased transcriptional activation, promoting the expression of the tanshinone\u2010biosynthetic gene\n                          <jats:italic>COPALYL DIPHOSPHATE SYNTHASE 1<\/jats:italic>\n                          (\n                          <jats:italic>SmCPS1<\/jats:italic>\n                          ) through its specific binding to the E2\u2010box of the\n                          <jats:italic>SmCPS1<\/jats:italic>\n                          promoter region.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Taken together, these findings open new possibilities for biotechnological strategies to boost tanshinone production, providing new insights into its metabolic regulation at the protein phosphorylation level.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.70825","type":"journal-article","created":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T04:44:52Z","timestamp":1766205892000},"page":"2496-2514","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Discovery of the roles of\n                    <scp>SmMAPK4<\/scp>\n                    in regulating tanshinone biosynthesis in\n                    <i>Salvia miltiorrhiza<\/i>"],"prefix":"10.1111","volume":"249","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6361-0491","authenticated-orcid":false,"given":"Jiahao","family":"Zhang","sequence":"first","affiliation":[{"name":"Clinical Metabolomics Center, School of Traditional Chinese Pharmacy China Pharmaceutical University  Nanjing 211198 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