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In C\n                          <jats:sub>3<\/jats:sub>\n                          plants, coordination between photosynthesis and stomatal conductance can be disrupted by mutations in Calvin\u2013Benson\u2013Bassham (CBB) cycle expression, but it is not clear whether the disruption of the C\n                          <jats:sub>4<\/jats:sub>\n                          carbon concentrating mechanism would have similar effects.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          In this study, it was hypothesized that perturbing the C\n                          <jats:sub>4<\/jats:sub>\n                          cycle, resulting in reduced carbon flux into the bundle sheath cells and overall net CO\n                          <jats:sub>2<\/jats:sub>\n                          assimilation rates, may alter stomatal regulation. To test this hypothesis, we performed gas exchange measurements on transgenic\n                          <jats:italic>Flaveria bidentis<\/jats:italic>\n                          plants carrying an antisense construct leading to reduced nicotinamide adenine dinucleotide phosphate (NADP)\u2010malic enzyme (NADP\u2010ME) activity.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Stomatal conductance was unaltered in the antisense plants at ambient CO\n                          <jats:sub>2<\/jats:sub>\n                          levels. However, C\n                          <jats:sub>i<\/jats:sub>\n                          was significantly increased. When measurements were instead performed at common C\n                          <jats:sub>i<\/jats:sub>\n                          , stomatal conductance was significantly higher in the antisense lines.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          These results demonstrate that disruption of the C\n                          <jats:sub>4<\/jats:sub>\n                          cycle via reduced NADP\u2010ME activity in\n                          <jats:italic>F. bidentis<\/jats:italic>\n                          leads to altered stomatal sensitivity to C\n                          <jats:sub>i<\/jats:sub>\n                          .\n                        <\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.71012","type":"journal-article","created":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T12:31:19Z","timestamp":1771417879000},"page":"1585-1597","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Antisense reduction in\n                    <scp>NADP<\/scp>\n                    \u2010\n                    <scp>ME<\/scp>\n                    in the\n                    <scp>\n                      C\n                      <sub>4<\/sub>\n                    <\/scp>\n                    species\n                    <i>Flaveria bidentis<\/i>\n                    alters stomatal sensitivity to intercellular [\n                    <scp>\n                      CO\n                      <sub>2<\/sub>\n                    <\/scp>\n                    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