{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:31:04Z","timestamp":1787315464149,"version":"build-2736575974"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T00:00:00Z","timestamp":1771113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T00:00:00Z","timestamp":1771113600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001381","name":"National Research Foundation Singapore","doi-asserted-by":"publisher","award":["NRF\u2010CRP22\u20102019\u20100001"],"award-info":[{"award-number":["NRF\u2010CRP22\u20102019\u20100001"]}],"id":[{"id":"10.13039\/501100001381","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>Stomatal development has emerged as a valuable model for studying developmental processes. Examining gene function along the stomatal lineage often requires gene perturbation in a controlled and cell\u2010stage\u2010specific manner, but this remains tedious without a dedicated genetic tool.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Here, we describe Stomatal XVE, a modular, two\u2010component XVE\u2010based inducible system that enables user\u2010controlled gene overexpression and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)\u2010based knockout at defined stomatal cell stages in\n                          <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                          . The system consists of a collection of estrogen\u2010responsive XVE driver lines under cell\u2010stage\u2010specific promoters and effector vectors responsive to activated XVE. This design simplifies cloning and allows users to scale their investigation.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          We validated the cell\u2010stage specificity and inducibility of the XVE driver lines and characterized key induction parameters. To test the system functionally, we employed it to study MAPKKK YODA and a pathogen effector AvrPtoB. While\n                          <jats:italic>YODA<\/jats:italic>\n                          overexpression reproduced known early\u2010 and late\u2010stage phenotypes, stage\u2010specific knockouts argued against its late\u2010stage role in guard cell (GC) differentiation. Furthermore,\n                          <jats:italic>AvrPtoB<\/jats:italic>\n                          expression during later stages triggered striking disruptions in GC morphology and viability, revealing cell\u2010type\u2010specific effects of the pathogen protein.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Overall, our Stomatal XVE system enables precise functional analysis of genes across defined stages of stomatal development and is particularly well suited for investigating genes with pleiotropic effects.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.71017","type":"journal-article","created":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T06:40:53Z","timestamp":1771224053000},"page":"1330-1347","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Stomatal\n                    <scp>XVE<\/scp>\n                    : an inducible system for cell\u2010stage\u2010specific gene expression and editing in the stomatal lineage"],"prefix":"10.1111","volume":"250","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3106-7331","authenticated-orcid":false,"given":"Senhao","family":"Kou","sequence":"first","affiliation":[{"name":"Department of Biological Sciences National University of Singapore  14 Science Drive 4 Singapore 117557 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9187-5050","authenticated-orcid":false,"given":"Li Cong","family":"Chua","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences National University of Singapore  14 Science Drive 4 Singapore 117557 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0133-4523","authenticated-orcid":false,"given":"Jie Quan","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences National University of Singapore  14 Science Drive 4 Singapore 117557 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8121-235X","authenticated-orcid":false,"given":"On Sun","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences National University of Singapore  14 Science Drive 4 Singapore 117557 Singapore"},{"name":"Research Center on Sustainable Urban Farming National University of Singapore  14 Science Drive 4 Singapore 117557 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1096014"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.58.032806.104023"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.106.081299"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiac028"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.10.013"},{"key":"e_1_2_9_7_1","article-title":"Stomatal development in the changing climate","volume":"151","author":"Chua LC","year":"2024","journal-title":"Development (Cambridge, UK)"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2022.04.040"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.103.027979"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.19.00454"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.04.018"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-023-01452-7"},{"key":"e_1_2_9_13_1","first-page":"2063","article-title":"Cell cycle \u2010dependent proteolysis in plants. 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