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However, potentially overlooked changes concern the pollen coat, which plays a role in different aspects of outcrossing strategy.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          To investigate this, we compared pollen coat thickness and pollen proteomes between selfing and outcrossing species in the\n                          <jats:italic>Arabidopsis<\/jats:italic>\n                          and\n                          <jats:italic>Capsella<\/jats:italic>\n                          genera, representing three independent selfing transitions.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Pollen coat area diminished with the age of the selfing transition, with significant reductions in\n                          <jats:italic>A. thaliana<\/jats:italic>\n                          and\n                          <jats:italic>Capsella rubella<\/jats:italic>\n                          , but not in\n                          <jats:italic>A. lyrata<\/jats:italic>\n                          (recent transition, no selfing syndrome). Moreover, specific differentially abundant pollen coat proteins were shared across selfer\u2013outcrosser contrasts more than expected by chance, even though the direction of changes was not consistent across the three comparisons. These specific proteins, while annotated as pathogen response due to their structure, are likely involved in pollen\u2013pistil interactions instead.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Overall, our findings reveal limited convergent evolution of pollen coat thickness and protein composition following selfing transitions. Our work identified a group of pollen coat proteins that are largely understudied, but whose abundance appears to be a hotspot for evolutionary changes.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.71167","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T06:42:46Z","timestamp":1776408166000},"page":"3460-3474","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The abundance of pollen coat small signaling proteins shows limited convergence between independent selfing transitions in\n                    <i>Arabidopsis<\/i>\n                    and\n                    <i>Capsella<\/i>"],"prefix":"10.1111","volume":"250","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4614-6673","authenticated-orcid":false,"given":"\u00d6mer","family":"\u0130lta\u015f","sequence":"first","affiliation":[{"name":"Department of Botany, Faculty of Science Charles University  CZ\u2010128 01 Prague Czech Republic"},{"name":"Max Planck Institute of Molecular Plant Physiology  Potsdam Science Park, Am M\u00fchlenberg 1 14476 Potsdam Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2640-4818","authenticated-orcid":false,"given":"Luciana","family":"Salomon","sequence":"additional","affiliation":[{"name":"Department of Botany, Faculty of Science Charles University  CZ\u2010128 01 Prague Czech 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