{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:40:47Z","timestamp":1789432847249,"version":"build-2803163510"},"reference-count":69,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>\n                          Thylakoid membranes (TM) in cyanobacteria and chloroplasts host the light\u2010dependent reactions of oxygenic photosynthesis.\n                          <jats:italic>Gloeobacterales<\/jats:italic>\n                          , the earliest\u2010diverging cyanobacterial lineage, lack TM and perform photosynthesis in the cytoplasmic membrane (CM), representing an ancestral state relative to other cyanobacteria (Phycobacteria). This study investigates the evolutionary origin of TM.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Phylogenomic analyses were performed across a phylogenetically diverse set of cyanobacteria, including extensive representation of basal lineages (\n                          <jats:italic>Gloeobacterales<\/jats:italic>\n                          ,\n                          <jats:italic>Thermostichales<\/jats:italic>\n                          ,\n                          <jats:italic>Gloeomargaritales<\/jats:italic>\n                          , and\n                          <jats:italic>Pseudanabaenales<\/jats:italic>\n                          ), as well as micro\u2010 and macrocyanobacteria, using orthologous proteins involved in membrane dynamics and photosystem II (PSII) assembly, together with structural modeling using AlphaFold3.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>We identified two candidate proteins associated with membrane trafficking that may contribute to TM biogenesis, including the SPFH (Stomatin, Prohibitin, Flotillin, en HflK\/C) family member Slr1106, proposed to have been acquired by lateral gene transfer. Analysis of 36 PSII assembly factors revealed modifications in late\u2010stage assembly, notably in manganese homeostasis. Structural changes in the YidC translocase may have facilitated the relocation of linear electron transfer components from the CM to TM.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Altogether, these phylogenetic and functional prediction analyses provide new insight into the molecular innovations that led to TM emergence, including membrane trafficking systems, PSII assembly changes, and protein targeting adaptations.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.71284","type":"journal-article","created":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T03:39:53Z","timestamp":1780717193000},"page":"1329-1343","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Exploring thylakoid emergence: evolution of membrane biogenesis and photosystem\n                    <scp>II<\/scp>\n                    assembly in early\u2010diverging cyanobacteria"],"prefix":"10.1111","volume":"251","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1861-4738","authenticated-orcid":false,"given":"Louise","family":"Hamb\u00fccken","sequence":"first","affiliation":[{"name":"InBioS\u2010PhytoSYSTEMS, Eukaryotic Phylogenomics University of Li\u00e8ge  Li\u00e8ge 4000 Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2388-6185","authenticated-orcid":false,"given":"Denis","family":"Baurain","sequence":"additional","affiliation":[{"name":"InBioS\u2010PhytoSYSTEMS, Eukaryotic Phylogenomics University of Li\u00e8ge  Li\u00e8ge 4000 Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3420-4488","authenticated-orcid":false,"given":"Luc","family":"Cornet","sequence":"additional","affiliation":[{"name":"InBioS\u2010PhytoSYSTEMS, Eukaryotic Phylogenomics University of Li\u00e8ge  Li\u00e8ge 4000 Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.4508"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02260"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2007.06.005"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0019625"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-421"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.180246"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-023-01940-w"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2014.00595"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-025-09100-w"},{"key":"e_1_2_9_12_1","article-title":"The GEN\u2010ERA toolbox: unified and reproducible workflows for research in microbial genomics","volume":"12","author":"Cornet L","year":"2023","journal-title":"GigaScience"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants9010018"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1832-y"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr367"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2015.08.010"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.14526"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2008.01470.x"},{"key":"e_1_2_9_19_1","first-page":"1090","article-title":"A conserved structure and function of the YidC homologous protein Slr1471 from Synechocystis sp. 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