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However, the cellular programs that coordinate periderm differentiation and secondary metabolism in perennial roots remain poorly understood.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Single\u2010cell RNA sequencing, together with spatial metabolome analyses, was employed to resolve cell\u2010type composition and transcriptional dynamics in\n                          <jats:italic>Salvia miltiorrhiza<\/jats:italic>\n                          roots at different developmental stages.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Based on Batch Integration via Precision Annotation and Consistency Testing\u2010integrated single\u2010cell data, the developmental trajectory from epidermal to peridermal lineages was reconstructed, revealing distinct transcriptional programs associated with diterpenoid biosynthesis. Spatial metabolome analyses confirmed that tanshinones were predominantly accumulated in cork cells of mature roots. Integrative analysis further indicated that SmCYP76AK5 participates in tanshinone biosynthesis.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>This cellular framework enables the prediction of metabolic activities in specific cell types and facilitates the inference of developmental differences between young and mature roots. It also provides a basis for integrating single\u2010cell data into models of secondary metabolism in perennial plants.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.71285","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T04:17:31Z","timestamp":1779423451000},"page":"1267-1282","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Cross\u2010stage single\u2010cell and spatial metabolome analyses reveal periderm specialization and tanshinone biosynthesis in\n                    <scp>\n                      <i>Salvia\u00a0miltiorrhiza<\/i>\n                    <\/scp>\n                    roots"],"prefix":"10.1111","volume":"251","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7091-4834","authenticated-orcid":false,"given":"Licheng","family":"Liu","sequence":"first","affiliation":[{"name":"Shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Botanical Garden, and Chenshan Science Research Center, CAS Center for Excellence in Molecular Plant Sciences (CEMPS), Chinese Academy of Sciences (CAS)  Shanghai 201602 China"},{"name":"University of Chinese Academy of Sciences  Shanghai 200032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3421-9539","authenticated-orcid":false,"given":"Lianchong","family":"Gao","sequence":"additional","affiliation":[{"name":"Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai JiaoTong University  Shanghai 200240 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