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Triploid production via 2\n                          <jats:italic>x<\/jats:italic>\n                          \u2009\u00d7\u20094\n                          <jats:italic>x<\/jats:italic>\n                          interploidy hybridization is an important strategy for obtaining seedless cultivar in some fruit tree crops including citrus. However, as the molecular mechanisms of postzygotic barrier in citrus remain unclear, triploid breeding efficiency is limited.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Here, we compared seed development in citrus 2\n                          <jats:italic>x<\/jats:italic>\n                          \u2009\u00d7\u20094\n                          <jats:italic>x<\/jats:italic>\n                          and 2\n                          <jats:italic>x<\/jats:italic>\n                          \u2009\u00d7\u20092\n                          <jats:italic>x<\/jats:italic>\n                          crosses. Embryo and endosperm developed normally in 2\n                          <jats:italic>x<\/jats:italic>\n                          \u2009\u00d7\u20092\n                          <jats:italic>x<\/jats:italic>\n                          crosses, while endosperm cellularization completely failed in 2\n                          <jats:italic>x<\/jats:italic>\n                          \u2009\u00d7\u20094\n                          <jats:italic>x<\/jats:italic>\n                          crosses, resulting in embryo arrest at the globular stage, demonstrating that impaired endosperm cellularization causes triploid embryo abortion.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Transcriptomic analysis of laser microdissection (LMD)\u2010captured endosperm at pre\u2010 and post\u2010cellularization stages revealed extensive transcriptional misregulation in 2\n                          <jats:italic>x<\/jats:italic>\n                          \u2009\u00d7\u20094\n                          <jats:italic>x<\/jats:italic>\n                          crosses, including persistent upregulation of type I MADS\u2010box genes (\n                          <jats:italic>CrAGLs<\/jats:italic>\n                          ) and the potential paternally expressed gene\n                          <jats:italic>CrbZIP<\/jats:italic>\n                          . Consistently, functional analyses showed that overexpression of\n                          <jats:italic>CrAGL62<\/jats:italic>\n                          ,\n                          <jats:italic>CrAGL80a<\/jats:italic>\n                          , or\n                          <jats:italic>CrbZIP<\/jats:italic>\n                          delayed endosperm cellularization in precocious Mini\u2010citrus (\n                          <jats:italic>Fortunella hindsii<\/jats:italic>\n                          ), while\n                          <jats:italic>cragl62<\/jats:italic>\n                          mutants exhibited severely impaired cellularization.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Our findings showed CrAGL62 and CrbZIP as key regulators of endosperm cellularization in citrus, providing potential targets for modulating endosperm development to improve citrus triploid breeding efficiency.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.71369","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T04:55:46Z","timestamp":1781758546000},"page":"2604-2619","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>CrAGL62<\/i>\n                    and potential paternally expressed gene\n                    <i>CrbZIP<\/i>\n                    contribute to paternal\u2010excess postzygotic barriers in citrus"],"prefix":"10.1111","volume":"251","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-4886-659X","authenticated-orcid":false,"given":"Jing\u2010Jing","family":"He","sequence":"first","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation &amp; Utilization of Horticultural Crops, College of Horticulture &amp; Forestry Sciences Huazhong Agricultural 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