{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T21:37:59Z","timestamp":1792618679689,"version":"4.5.0"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>\n                          Pyrenoid\u2010based CO\n                          <jats:sub>2<\/jats:sub>\n                          \u2010concentrating mechanisms (pCCMs) boost photosynthesis by delivering elevated levels of CO\n                          <jats:sub>2<\/jats:sub>\n                          to Ribulose\u20101,5\u2010bisphosphate carboxylase\/oxygenase. Pyrenoids are often surrounded by a starch sheath thought to enhance pCCM efficiency, but little is known about how the sheath is formed. Here, we have assessed the role of the pyrenoid\u2010associated protein EPOS1 in starch sheath formation in\n                          <jats:italic>Chlamydomonas reinhardtii<\/jats:italic>\n                          .\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Using an\n                          <jats:italic>epos1<\/jats:italic>\n                          mutant, we assessed the role of EPOS1 in starch sheath morphology by fluorescence and electron microscopy, and its impact on pCCM function by CO\n                          <jats:sub>2<\/jats:sub>\n                          uptake and growth assays. We determined potential interactors of EPOS1 via TurboID proximity labelling.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          In the absence of EPOS1, cells fail to correctly assemble the starch sheath, have lower affinity for inorganic carbon and grow slower under CO\n                          <jats:sub>2<\/jats:sub>\n                          \u2010limiting conditions. EPOS1 localises to the starch sheath and is physically close to a range of starch metabolism enzymes and other potential scaffolding proteins. Finally, we show that EPOS1 associates with starch in the land plant\n                          <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                          .\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>EPOS1 may act as a pyrenoid\u2010specific regulator or scaffold for starch metabolism enzymes. EPOS1 could contribute to ongoing efforts to build a functional pyrenoid in a land plant.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.71373","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T03:24:50Z","timestamp":1783481090000},"page":"2518-2531","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<scp>EPOS1<\/scp>\n                    is required for normal pyrenoid starch sheath formation and efficiency of the\n                    <scp>\n                      CO\n                      <sub>2<\/sub>\n                    <\/scp>\n                    \u2010concentrating mechanism in\n                    <i>Chlamydomonas reinhardtii<\/i>"],"prefix":"10.1111","volume":"251","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4839-418X","authenticated-orcid":false,"given":"Liat","family":"Adler","sequence":"first","affiliation":[{"name":"Centre for Novel Agriculture Products, Department of Biology University of York  York YO10 5DD UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4148-035X","authenticated-orcid":false,"given":"Chun Sing","family":"Lau","sequence":"additional","affiliation":[{"name":"Centre for Novel Agriculture Products, Department of Biology University of York  York YO10 5DD UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2049-1882","authenticated-orcid":false,"given":"Lara","family":"Esch","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Institute of Molecular Plant Sciences University of Edinburgh  Edinburgh EH9 3BF UK"},{"name":"Centre for Engineering Biology University of Edinburgh  Edinburgh EH9 3BF UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7040-5770","authenticated-orcid":false,"given":"Ousmane","family":"Dao","sequence":"additional","affiliation":[{"name":"Centre for Novel Agriculture Products, Department of Biology University of York  York YO10 5DD UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2206-2045","authenticated-orcid":false,"given":"James","family":"Barrett","sequence":"additional","affiliation":[{"name":"Centre for Novel Agriculture Products, Department of Biology University of York  York YO10 5DD UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4792-4676","authenticated-orcid":false,"given":"Sebastian","family":"Grant","sequence":"additional","affiliation":[{"name":"Centre for Novel Agriculture Products, Department of Biology University of York  York YO10 5DD UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1301-2452","authenticated-orcid":false,"given":"Gary","family":"Yates","sequence":"additional","affiliation":[{"name":"Centre for Novel Agriculture Products, Department of Biology University of York  York YO10 5DD UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7255-872X","authenticated-orcid":false,"given":"Alistair J.","family":"McCormick","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Institute of Molecular Plant Sciences University of Edinburgh  Edinburgh EH9 3BF UK"},{"name":"Centre for Engineering Biology University of Edinburgh  Edinburgh EH9 3BF UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1440-3233","authenticated-orcid":false,"given":"Luke C. 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