{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T17:29:22Z","timestamp":1787246962830,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>\n                          Tree peony (\n                          <jats:italic>Paeonia suffruticosa<\/jats:italic>\n                          ) is an important garden plant with high ornamental and economic value. Bud endodormancy release is prerequisite for bud break, which affects its flowering time and quality, and activating gibberellin (GA) signaling is a key factor in this process. The DELLA protein PsRGL1 negatively regulates bud break, but its interacting transcription factors and their roles remain largely unexplored.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Here, we identified a direct interacting partner of PsRGL1, PsWRKY71, using yeast two\u2010hybrid, pull\u2010down, and luciferase complementation assays.\n                          <jats:italic>PsWRKY71<\/jats:italic>\n                          was significantly induced by both chilling and exogenous GA\n                          <jats:sub>3<\/jats:sub>\n                          treatments. Functional studies showed that\n                          <jats:italic>PsWRKY71<\/jats:italic>\n                          promoted bud break and upregulated the expression of bud\u2010break\u2010related genes. RNA\u2010seq and subsequent analyses revealed that PsWRKY71 directly bound W\u2010box elements of the\n                          <jats:italic>PsCYCD6.1<\/jats:italic>\n                          promoter to activate its expression. Notably, PsRGL1 inhibited the DNA\u2010binding ability and transactivation activity of PsWRKY71. Furthermore, overexpression of\n                          <jats:italic>PsCYCD6.1<\/jats:italic>\n                          increased the proportion of S\u2010phase cells and subsequently accelerated bud burst.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Our results demonstrated that PsWRKY71 acts as a positive regulator of bud break by accelerating cell proliferation and integrating it into the GA signaling pathway by interacting with PsRGL1.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          These findings elucidate a GA pathway, PsRGL1\u2010PsWRKY71\u2010\n                          <jats:italic>PsCYCD6.1<\/jats:italic>\n                          , which enriches the mechanism of bud break in tree peony.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.71434","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T03:28:28Z","timestamp":1784172508000},"page":"3332-3346","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>PsWRKY71<\/scp>\n                    acts as a\n                    <scp>GA<\/scp>\n                    signaling component to promote bud break in tree peony (\n                    <i>Paeonia suffruticosa<\/i>\n                    )"],"prefix":"10.1111","volume":"251","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9530-2732","authenticated-orcid":false,"given":"Linqiang","family":"Gao","sequence":"first","affiliation":[{"name":"College of Life Sciences Qingdao Agricultural University  Qingdao 266109 China"},{"name":"University Key Laboratory of Plant Biotechnology in Shandong Province  Qingdao 266109 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Laboratory of Plant Biotechnology in Shandong Province  Qingdao 266109 China"},{"name":"Shandong Provincial Key Laboratory of Microbial Resource Exploration and Innovative Utilization  Qingdao 266109 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengdi","family":"Cui","sequence":"additional","affiliation":[{"name":"College of Life Sciences Qingdao Agricultural University  Qingdao 266109 China"},{"name":"University Key Laboratory of Plant Biotechnology in Shandong Province  Qingdao 266109 China"},{"name":"Shandong Provincial Key Laboratory of Microbial Resource Exploration and Innovative Utilization  Qingdao 266109 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanchao","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Life Sciences Qingdao Agricultural University  Qingdao 266109 China"},{"name":"University Key Laboratory of Plant Biotechnology in Shandong Province  Qingdao 266109 China"},{"name":"Shandong Provincial Key Laboratory of Microbial Resource Exploration and Innovative Utilization  Qingdao 266109 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunying","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Life Sciences Qingdao Agricultural University  Qingdao 266109 China"},{"name":"University Key Laboratory of Plant Biotechnology in Shandong Province  Qingdao 266109 China"},{"name":"Shandong Provincial Key Laboratory of Microbial Resource Exploration and Innovative Utilization  Qingdao 266109 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3280-7805","authenticated-orcid":false,"given":"Shupeng","family":"Gai","sequence":"additional","affiliation":[{"name":"College of Life Sciences Qingdao Agricultural University  Qingdao 266109 China"},{"name":"University Key Laboratory of Plant Biotechnology in Shandong Province  Qingdao 266109 China"},{"name":"Shandong Provincial Key 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