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While the role of clathrin in endocytosis at the PM is well established, its function at TGN\/EE, presumably in late secretion (trafficking from the TGN\/EE to the cell surface) or en route to the vacuole, is debated. Similarly debated are potential homeostatic mechanisms balancing the trafficking routes, especially endocytosis and late secretion.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          We address these questions in\n                          <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                          using conditional silencing of\n                          <jats:italic>CLATHRIN HEAVY CHAIN<\/jats:italic>\n                          (\n                          <jats:italic>CHC<\/jats:italic>\n                          ), conditional overexpression of the clathrin uncoating factor AUXILIN\u2010LIKE1, and secretory mutants.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          <jats:italic>CHC<\/jats:italic>\n                          silencing interferes with trafficking of cargoes destined for the apoplast and the PM, supporting a function of clathrin in late secretion. The secretory cargoes become abnormally rerouted from the TGN\/EE to the vacuole. Unlike\n                          <jats:italic>CHC<\/jats:italic>\n                          silencing, overexpression of AUXILIN\u2010LIKE1 selectively inhibits clathrin\u2010mediated endocytosis while secretion continues normally at early points of induction. Conversely, secretory mutants exhibit a reduced PM recruitment of clathrin, and variably, of the TPLATE endocytic component.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Together, our data show a role of clathrin in secretion and suggest secretion as a fundamental trafficking process to which endocytosis is adjusted by a weak homeostatic mechanism.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.71454","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T03:29:23Z","timestamp":1784604563000},"page":"3401-3417","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The role of clathrin in post\u2010Golgi secretion in plant cells"],"prefix":"10.1111","volume":"251","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6463-5257","authenticated-orcid":false,"given":"Maciek","family":"Adamowski","sequence":"first","affiliation":[{"name":"Institute of Science and Technology Austria  Am Campus 1 3400 Klosterneuburg Austria"},{"name":"Intercollegiate Faculty of Biotechnology University of Gda\u0144sk and Medical University of Gda\u0144sk  Abrahama 58 80\u2010307 Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5760-0900","authenticated-orcid":false,"given":"Adam","family":"Gackowski","sequence":"additional","affiliation":[{"name":"Intercollegiate Faculty of Biotechnology University of Gda\u0144sk and Medical University of Gda\u0144sk  Abrahama 58 80\u2010307 Gda\u0144sk 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