{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T06:04:45Z","timestamp":1789625085242,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["CA\u2010R\u2010STA\u20107132\u2010H"],"award-info":[{"award-number":["CA\u2010R\u2010STA\u20107132\u2010H"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000121","name":"Division of Mathematical Sciences","doi-asserted-by":"publisher","award":["1853698"],"award-info":[{"award-number":["1853698"]}],"id":[{"id":"10.13039\/100000121","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>Cell polarity and tip growth rely on the dynamic spatial organization of signaling and structural components. Quantitative characterization of these spatiotemporal dynamics is critical for understanding polarized cell growth, yet manual quantification is labor\u2010intensive and existing computational tools often lack the flexibility and robustness needed to analyze molecular and structural dynamics in tip\u2010growing cells.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Tip Quantification (TipQuant) identifies the cell apex by detecting the site of maximum expansion and automatically quantifies fluorescence distribution along the plasma membrane and within the apical cytoplasm from live\u2010cell imaging data, enabling analysis of the spatiotemporal dynamics of molecular and structural components in tip\u2010growing cells.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          TipQuant accurately identified cell apices and quantified the spatiotemporal behavior of fluorescently labeled proteins and cellular structures in\n                          <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                          pollen tubes and\n                          <jats:italic>Fusarium graminearum<\/jats:italic>\n                          hyphae, reproducing manual measurements while reducing user bias and improving efficiency, consistency, and analytical flexibility. The tool also revealed a strong positive correlation between rho\u2010like GTPase from plants activity and apical Ca\n                          <jats:sup>2+<\/jats:sup>\n                          influx in Arabidopsis pollen tubes, demonstrating its utility for analyzing dynamic cellular processes.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>TipQuant is a robust analytical tool for quantifying spatiotemporal dynamics in tip\u2010growing cells, providing a flexible alternative to manual image analysis and enabling studies of the molecular mechanisms underlying polarized growth.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.71474","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T08:47:22Z","timestamp":1787215642000},"page":"926-935","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>TipQuant<\/scp>\n                    : a robust algorithm for quantitative analysis of spatiotemporally dynamic activities in tip\u2010growing cells"],"prefix":"10.1111","volume":"252","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0858-527X","authenticated-orcid":false,"given":"Jingzhe","family":"Guo","sequence":"first","affiliation":[{"name":"Institute for Integrative Genome Biology and Department of Botany and Plant Sciences University of California  Riverside CA 92521 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian","family":"Le Gouic","sequence":"additional","affiliation":[{"name":"Capgemini Invent  92130 Issy\u2010les\u2010Moulineaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00e9mi","family":"Rosenthal","sequence":"additional","affiliation":[{"name":"Quantmetry  52, rue d'Anjou 75008 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ailing","family":"Zou","sequence":"additional","affiliation":[{"name":"Metabolomics Research Center, Haixia Institute of Science and Technology Fujian Agriculture and Forestry University  Fuzhou 350002 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Quantitative Synthetic Biology Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences  Shenzhen 518055 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2840-8484","authenticated-orcid":false,"given":"Nicolas","family":"Brunel","sequence":"additional","affiliation":[{"name":"Capgemini Invent  92130 Issy\u2010les\u2010Moulineaux France"},{"name":"ENSIIE &amp; Laboratoire de Math\u00e9matiques et Mod\u00e9lisation d'Evry Universit\u00e9 Paris Saclay  91025 Evry France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7482-6540","authenticated-orcid":false,"given":"Zhenbiao","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute for Integrative Genome Biology and Department of Botany and Plant Sciences University of California  Riverside CA 92521 USA"},{"name":"Institute of Emerging Agricultural Technology Shenzhen University of Advanced Technology Shenzhen 518107 Guangdong China"},{"name":"Faculty of Synthetic Biology Shenzhen University of Advanced Technology Shenzhen 518107 Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1420-2696","authenticated-orcid":false,"given":"Xinping","family":"Cui","sequence":"additional","affiliation":[{"name":"Institute for Integrative Genome Biology and Department of Statistics University of California  Riverside CA 92521 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"e_1_2_9_2_1","article-title":"AMEBaS: automatic midline extraction and background subtraction of ratiometric fluorescence time\u2010lapses of polarized single cells","volume":"196","author":"Badain R","year":"2023","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-0672-8_20"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.59.032607.092921"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.58768"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erx032"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81278-7"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.152.5.1019"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200409140"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/mp\/sst070"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiac020"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eraa134"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-0672-8_21"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/mp\/sst103"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1062429"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E05-05-0409"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.039180"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.010359"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.70691"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcad020"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200801086"},{"key":"e_1_2_9_22_1","first-page":"1731","article-title":"Control of pollen tube tip growth by a Rop GTPase\u2010dependent pathway that leads to tip\u2010localized calcium influx","volume":"11","author":"Li H","year":"1999","journal-title":"Plant Cell"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01452-0"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.25080\/Majora-92bf1922-00a"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07882"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1996.0060"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00497-018-00351-8"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2011.06.004"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v109.i02"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-4020-2_4"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.108.060277"},{"key":"e_1_2_9_33_1","doi-asserted-by":"crossref","unstructured":"TamboAL BhanuB LuoN RongD WangF CraddockC LavagiI YangZ.2020.TipQAD: an automated tool for quantifying apical fluorescence dynamics in tip\u2010growing cells.bioRxiv: 2020.2003.2001.960591.","DOI":"10.1101\/2020.03.01.960591"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00285-019-01396-7"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar6464"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.847671"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0910811106"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.23.090506.123233"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.16935"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2021.02.030"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erq073"}],"container-title":["New Phytologist"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nph.71474","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/nph.71474","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nph.71474","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T05:52:40Z","timestamp":1789624360000},"score":1,"resource":{"primary":{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/10.1111\/nph.71474"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,20]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/nph.71474"],"URL":"https:\/\/doi.org\/10.1111\/nph.71474","archive":["Portico"],"relation":{},"ISSN":["0028-646X","1469-8137"],"issn-type":[{"value":"0028-646X","type":"print"},{"value":"1469-8137","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,20]]},"assertion":[{"value":"2026-05-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}