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We identified\n                          <jats:italic>PeuPLD\u03b64<\/jats:italic>\n                          , a new gene arising from tandem duplication in\n                          <jats:italic>P. euphratica<\/jats:italic>\n                          , and evaluated its effects on root growth and drought\u2010related performance.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Phylogenetic and promoter\u2010reporter analyses, enzyme assays, and subcellular localization characterized\n                          <jats:italic>PeuPLD\u03b64<\/jats:italic>\n                          divergence. Its effects were evaluated in transgenic\n                          <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                          ,\n                          <jats:italic>P. alba<\/jats:italic>\n                          var.\n                          <jats:italic>pyramidalis<\/jats:italic>\n                          , wheat, and rice. Phosphatidic acid (PA) and hormone measurements, reporter imaging, transcriptomics, protein\u2013lipid overlay, dual\u2010luciferase, and electrophoretic mobility shift assays investigated the mechanism.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Compared with its paralog\n                          <jats:italic>PeuPLD\u03b63<\/jats:italic>\n                          ,\n                          <jats:italic>PeuPLD\u03b64<\/jats:italic>\n                          showed drought\u2010inducible root expression, higher enzymatic activity, and additional nuclear\u2010associated localization.\n                          <jats:italic>PeuPLD\u03b64<\/jats:italic>\n                          overexpression promoted root growth across species and was associated with increased PA accumulation in root\u2010tip tissues, elevated auxin (IAA) and cytokinin (CK) contents, enhanced IAA and CK reporter responses, and an increased IAA\u2009:\u2009CK ratio. NAC097 was identified as a PA\u2010associated candidate regulator that bound and activated the LONELY GUY 2 (\n                          <jats:italic>LOG2<\/jats:italic>\n                          ) and IIOPENTENYLTRANSFERASE 4 (\n                          <jats:italic>IPT4<\/jats:italic>\n                          ) promoters.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          These findings indicate that functional divergence of a newly duplicated\n                          <jats:italic>PLD\u03b6<\/jats:italic>\n                          gene contributed to root\u2010related adaptation in\n                          <jats:italic>P. euphratica<\/jats:italic>\n                          . The PA\u2010associated NAC097\u2013\n                          <jats:italic>LOG2<\/jats:italic>\n                          \/\n                          <jats:italic>IPT4<\/jats:italic>\n                          branch provides a candidate link between lipid signaling and CK regulation, while\n                          <jats:italic>PeuPLD\u03b64<\/jats:italic>\n                          may offer a resource for improving drought\u2010related traits in crops.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.71511","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T03:26:27Z","timestamp":1787109987000},"page":"685-704","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evolutionary origin of a new gene drives root growth in one desert poplar"],"prefix":"10.1111","volume":"252","author":[{"given":"Chengyu","family":"Gao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Herbage Improvement and Grassland Agro\u2010Ecosystems, College of Ecology Lanzhou University  Lanzhou 730000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotao","family":"Bai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Herbage Improvement and Grassland Agro\u2010Ecosystems, College of Ecology Lanzhou University  Lanzhou 730000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Wei","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Herbage Improvement and Grassland Agro\u2010Ecosystems, College of Ecology Lanzhou University  Lanzhou 730000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuemin","family":"Shao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Herbage 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