{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T06:04:47Z","timestamp":1789625087694,"version":"build-2803163510"},"reference-count":77,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>\n                          Powdery mildew (PM) of strawberry (\n                          <jats:italic>Fragaria \u00d7 ananassa<\/jats:italic>\n                          ) is a prevalent fungal disease affecting all aboveground tissues, especially leaves. Currently, there are few germplasm resources of strawberry resistant to PM, and the resistance mechanism remains unclear.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          This study constructed a BC\n                          <jats:sub>1<\/jats:sub>\n                          F\n                          <jats:sub>1<\/jats:sub>\n                          segregating population by crossing the highly resistant \u2018Parajatan\u2019 with the susceptible \u2018Benihoppe\u2019. Using multi\u2010omics approaches, including RNA\u2010seq, QTL\u2010seq, and metabolomics analysis, a key gene\n                          <jats:italic>FaNAC29<\/jats:italic>\n                          was identified.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Subcellular localization revealed\n                          <jats:italic>FaNAC29<\/jats:italic>\n                          nuclear expression, and transcriptional activation assays confirmed its activation activity. Notably, functional assays revealed that\n                          <jats:italic>FaNAC29<\/jats:italic>\n                          confers enhanced PM resistance in strawberry seedlings. We further identified the downstream target gene\n                          <jats:italic>FaPR4<\/jats:italic>\n                          and the interacting protein\n                          <jats:italic>FaZHD11<\/jats:italic>\n                          , using DAP\u2010seq and Y2H assays, respectively. We characterized\n                          <jats:italic>FaPR4<\/jats:italic>\n                          as a direct downstream target of\n                          <jats:italic>FaNAC29<\/jats:italic>\n                          , which enhances strawberry PM resistance via the modulation of chitinase activity.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>These findings provide a theoretical basis for breeding strawberry varieties with both excellent commercial traits and PM resistance.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  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