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Although proline accumulation is a major adaptive response to water deficit, how drought\u2010responsive regulatory networks control proline biosynthesis in woody grasses remains poorly understood.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Here, we combined genetic manipulation, molecular assays, and physiological analyses to investigate the function of the DlamiR156\u2010DlaSPL60 module in Ma bamboo (\n                          <jats:italic>Dendrocalamus latifloru<\/jats:italic>\n                          s Munro).\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          We show that DlamiR156 acts as a negative regulator of drought tolerance by repressing DlaSPL60 via transcript cleavage, whereas drought\u2010induced reduction of DlamiR156 releases this repression; DlaSPL60 directly binds to the\n                          <jats:italic>DlaP5CS1<\/jats:italic>\n                          promoter and activates its transcription, thereby promoting proline accumulation.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Our findings establish a mechanistic link between the conserved miR156\u2013squamosa promoter\u2010binding protein\u2010like regulatory module and proline biosynthesis in bamboo, providing new insights into how post\u2010transcriptional regulation coordinates metabolic reprogramming during drought adaptation in perennial woody grasses.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  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