{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T04:14:17Z","timestamp":1788408857679,"version":"build-2803163510"},"reference-count":112,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Norges Forskningsr\u00e5d","doi-asserted-by":"publisher","award":["276053"],"award-info":[{"award-number":["276053"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>\n                          In plants, the epigenetic phenomenon of parent\u2010of\u2010origin allele\u2010specific expression occurs mainly in the triploid endosperm. Although well studied in inbreeding\n                          <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                          , genomic imprinting has been less investigated in outcrossers. In order to investigate a wider role of parental\u2010specific allelic expression, we have analyzed imprinting in whole seeds of the obligate outbreeder\n                          <jats:italic>Arabidopsis arenosa<\/jats:italic>\n                          .\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>High\u2010throughput analysis of imprinting in outbreeding species is hampered by the lack of reference genomes and available sequenced accessions. High degree of allelic variation in outbreeding species may also limit the analysis to loci with less variation. We developed a reference\u2010independent pipeline to detect parental\u2010specific reads.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Using different accessions in reciprocal crosses, we detected more than 70 paternally biased imprinted genes and &gt;\u2009500 maternally biased genes. Paternally biased genes showed major enrichment for proteins with ubiquitin protein transferase and ligase activity. Maternally biased genes were enriched for protein pathways directly counteracting paternally enriched genes.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Here, we demonstrate an alignment\u2010free protocol to identify imprinted genes that may be successfully applied for imprinting studies in other highly heterozygous outcrossing species. Our results suggest a unique role of genomic imprinting affecting post\u2010transcriptional gene regulation in outbreeding\n                          <jats:italic>A. arenosa<\/jats:italic>\n                          .\n                        <\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.71551","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T03:36:56Z","timestamp":1788406616000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Parent\u2010of\u2010origin specific allelic expression in outbreeding\n                    <i>Arabidopsis arenosa<\/i>\n                    identifies antagonistic parental enrichment in protein degradation pathways"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3763-415X","authenticated-orcid":false,"given":"Karina S.","family":"Hornslien","sequence":"first","affiliation":[{"name":"Section for Genetics and Evolutionary Biology, Department of Biosciences University of Oslo  0316 Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3414-7049","authenticated-orcid":false,"given":"Ida V.","family":"Myking","sequence":"additional","affiliation":[{"name":"Section for Genetics and Evolutionary Biology, Department of Biosciences University of Oslo  0316 Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1517-9096","authenticated-orcid":false,"given":"Katrine N.","family":"Bjerkan","sequence":"additional","affiliation":[{"name":"Section for Genetics and Evolutionary Biology, Department of Biosciences University of Oslo  0316 Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9481-8396","authenticated-orcid":false,"given":"Anders K.","family":"Krabber\u00f8d","sequence":"additional","affiliation":[{"name":"Section for Genetics and Evolutionary Biology, Department of Biosciences University of Oslo  0316 Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6912-2925","authenticated-orcid":false,"given":"Jason R.","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Computer Science and Information Technology Hood College  Frederick MD 21701 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3898-6277","authenticated-orcid":false,"given":"Paul E.","family":"Grini","sequence":"additional","affiliation":[{"name":"Section for Genetics and Evolutionary Biology, Department of Biosciences University of Oslo  0316 Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1111\/j.1365-313X.2005.02467.x","article-title":"Arabidopsis CUL3A and CUL3B genes are essential for normal embryogenesis","volume":"43","author":"Alexis T","year":"2005","journal-title":"The Plant journal: for cell and molecular biology"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1038\/349084a0","article-title":"The mouse insulin\u2010like growth factor type\u20102 receptor is imprinted and closely linked to the Tme locus","volume":"349","author":"Barlow DP","year":"1991","journal-title":"Nature"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1038\/nrg776","article-title":"The evolution of plant sexual diversity","volume":"3","author":"Barrett SCH","year":"2002","journal-title":"Nature Reviews. 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