{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T04:14:49Z","timestamp":1788408889372,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["325443116"],"award-info":[{"award-number":["325443116"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>RNA interference (RNAi) shows great potential to protect crops against fungal diseases, yet reported protection efficiencies vary greatly, and our understanding of the factors responsible for this variance remains limited.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>In this meta\u2010analysis, we evaluated 89 studies that compare the efficiency of host\u2010induced gene silencing (HIGS) and spray\u2010induced gene silencing (SIGS) in controlling fungal diseases, focusing on biotrophic, hemibiotrophic, and necrotrophic fungi, the use of formulations, and the dsRNA design as explanatory factors for differences between reported efficiency values.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Our results indicate that SIGS is slightly more effective, particularly against biotrophs. Surprisingly, SIGS studies using formulations did not outperform those applying naked dsRNA. We also assessed parameters of RNA design. Differences in dsRNA length and the number of constructs and number of targets showed no consistent significant effect on resistance in either HIGS or SIGS. However, HIGS studies reported significantly higher efficiency when targeting genes closer to the 3\u2032 end and SIGS when targeting genes closer to the 5\u2032 end.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>We discuss potential reasons for the reported patterns, such as variability in dsRNA uptake mechanisms, intercellular trafficking, and Dicer processing, and conclude that more research is needed to understand the biological mechanisms determining RNAi efficiency for fungal control.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.71552","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T03:43:52Z","timestamp":1788407032000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Efficiency of\n                    <scp>RNAi<\/scp>\n                    \u2010based gene silencing in fungi: a meta\u2010analysis"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9156-9694","authenticated-orcid":false,"given":"Patrick","family":"Barth","sequence":"first","affiliation":[{"name":"Plant RNA Biotechnology University of Regensburg  Universit\u00e4tsstrasse 31 Regensburg 93053 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6459-9828","authenticated-orcid":false,"given":"Jakob","family":"Drumm","sequence":"additional","affiliation":[{"name":"Plant RNA Biotechnology University of Regensburg  Universit\u00e4tsstrasse 31 Regensburg 93053 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra Erika","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Institute of Phytomedicine (360), University of Hohenheim  Otto\u2010Sander\u2010Strasse 5 70599 Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6255-9059","authenticated-orcid":false,"given":"Florian","family":"Hartig","sequence":"additional","affiliation":[{"name":"Theoretical Ecology University of Regensburg  Universit\u00e4tsstrasse 31 93053 Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3536-5344","authenticated-orcid":false,"given":"Aline","family":"Koch","sequence":"additional","affiliation":[{"name":"Plant RNA Biotechnology University of Regensburg  Universit\u00e4tsstrasse 31 Regensburg 93053 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1007\/s12038-020-00098-3","article-title":"Post\u2010transcriptional gene silencing: Basic concepts and applications","volume":"45","author":"Ashfaq MA","year":"2020","journal-title":"Journal of Biosciences"},{"key":"e_1_2_8_3_1","unstructured":"Barto\u0144K.2024.MuMIn: multi\u2010model inference. 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