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Non\u2010iron metal substrates of IRT1 were recently shown to regulate IRT1 cell surface levels by endocytosis and vacuolar degradation.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>TurboID\u2010based proximity labeling was recently developed to detect protein\u2013protein interactions and thus shed light on the intricate regulation of proteins in living cells. Although TurboID\u2010based proximity labeling technology has been successfully established in mammals, its application in plant systems remains limited, especially for highly hydrophobic multispan transmembrane proteins.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Here, we established TurboID for proximity labeling of IRT1 and identified 494 IRT1\u2010specific proximal proteins, including the previously reported FYVE1 IRT1\u2010interacting protein. To showcase the biological relevance of identified IRT1 proximal proteins, we further characterized the NHX5 Na\n                          <jats:sup>+<\/jats:sup>\n                          (K\n                          <jats:sup>+<\/jats:sup>\n                          )\/H\n                          <jats:sup>+<\/jats:sup>\n                          antiporter and the RGLG2 E3 ubiquitin ligase. We validated both IRT1 proximal proteins as genuine IRT1 partners using several orthogonal assays. We also uncovered the contribution of NHX\u2010type antiporters and RGLG\u2010type E3 ligases in plant responses to non\u2010iron metal nutrition and IRT1 endocytosis.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Overall, our work further highlights the power of TurboID to identify new interacting proteins and showcases its application to plant transporters.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.71560","type":"journal-article","created":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T07:11:52Z","timestamp":1789283512000},"page":"1545-1564","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>TurboID<\/scp>\n                    \u2010based proteomic profiling reveals proxitome of the\n                    <scp>IRT1<\/scp>\n                    metal transporter and new insight into\n                    <scp>IRT1<\/scp>\n                    endocytosis"],"prefix":"10.1111","volume":"252","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7013-9082","authenticated-orcid":false,"given":"Liza","family":"Pellegrin","sequence":"first","affiliation":[{"name":"Laboratoire de Recherche en Sciences V\u00e9g\u00e9tales (LRSV) Universit\u00e9 de Toulouse, CNRS, Toulouse\u2010INP  31320 Auzeville\u2010Tolosane France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1390-2404","authenticated-orcid":false,"given":"Steven","family":"Fanara","sequence":"additional","affiliation":[{"name":"Laboratoire de Recherche en Sciences V\u00e9g\u00e9tales (LRSV) Universit\u00e9 de Toulouse, CNRS, Toulouse\u2010INP  31320 Auzeville\u2010Tolosane 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Auzeville\u2010Tolosane France"},{"name":"Infrastructure Nationale de Prot\u00e9omique, ProFI, UAR 2048  31400 Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5392-9732","authenticated-orcid":false,"given":"Nathalie","family":"Leonhardt","sequence":"additional","affiliation":[{"name":"Institut de Biosciences et Biotechnologies d'Aix\u2010Marseille (BIAM), Universit\u00e9 Aix\u2010Marseille, CEA, CNRS  13115 Saint\u2010Paul Lez Durance France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5297-6329","authenticated-orcid":false,"given":"Val\u00e9rie","family":"Cotelle","sequence":"additional","affiliation":[{"name":"Laboratoire de Recherche en Sciences V\u00e9g\u00e9tales (LRSV) Universit\u00e9 de Toulouse, CNRS, Toulouse\u2010INP  31320 Auzeville\u2010Tolosane 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