{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T07:45:12Z","timestamp":1792050312590,"version":"4.4.0"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GR 5871\/2\u20101"],"award-info":[{"award-number":["GR 5871\/2\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2026,11]]},"abstract":"Summary<\/jats:title>\n \n \n \n \n Nitrous oxide (N\n 2<\/jats:sub>\n O) contributes substantially to climate change and stratospheric ozone degradation, yet large uncertainties in its global budget point to overlooked sources. Growing evidence suggests plants produce N\n 2<\/jats:sub>\n O, but the mechanisms remain poorly constrained.\n <\/jats:p>\n <\/jats:list-item>\n \n \n For the first time, we resolved organ\u2010specific N\n 2<\/jats:sub>\n O formation in roots and shoots of aseptically cultivated chicory (\n Cichorium intybus<\/jats:italic>\n ) under light and dark conditions, combining\n 15<\/jats:sup>\n N\u2010labelling (\n 15<\/jats:sup>\n NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n \/\n 15<\/jats:sup>\n NH\n 4<\/jats:sub>\n +<\/jats:sup>\n ) with position\u2010specific isotope analysis to identify N\n 2<\/jats:sub>\n O formation pathways.\n <\/jats:p>\n <\/jats:list-item>\n \n \n All cultures produced N\n 2<\/jats:sub>\n O under dark conditions, whereas illuminated shoots showed reduced production or net uptake, indicating photosynthetically driven suppression or internal consumption. Roots supplied with\n 15<\/jats:sup>\n NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n exhibited relatively symmetric\n 15<\/jats:sup>\n N enrichment at the \u03b1\u2010 and \u03b2\u2010positions of emitted N\n 2<\/jats:sub>\n O (compared to shoots), consistent with a reductive pathway that may involve nitric oxide as an intermediate. By contrast, shoots showed asymmetric intramolecular\n 15<\/jats:sup>\n N enrichment exclusively under dark conditions with\n 15<\/jats:sup>\n NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n , indicating a NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n \u2010dependent pathway mechanistically distinct from that operating in roots. No significant\n 15<\/jats:sup>\n N incorporation was detected in any other shoot treatment.\n <\/jats:p>\n <\/jats:list-item>\n \n \n These organ\u2010specific, light\u2010dependent dynamics are absent from global N\n 2<\/jats:sub>\n O budgets and vegetation models. Our findings reveal unrecognized complexity in plant N\n 2<\/jats:sub>\n O exchange and provide a mechanistic framework for incorporating vegetation processes into future N\n 2<\/jats:sub>\n O budget assessments.\n <\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>","DOI":"10.1111\/nph.71565","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T23:40:21Z","timestamp":1789515621000},"page":"1432-1444","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Insights into plant\u2010part\u2010specific\n \n N\n 2<\/sub>\n O\n <\/scp>\n production in roots and shoots of chicory (\n Cichorium intybus<\/i>\n ) using stable isotope labelling"],"prefix":"10.1111","volume":"252","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4416-1610","authenticated-orcid":false,"given":"Moritz","family":"Schroll","sequence":"first","affiliation":[{"name":"Institute of Earth Sciences Heidelberg University Heidelberg 69120 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4420-7923","authenticated-orcid":false,"given":"Maurice","family":"Maas","sequence":"additional","affiliation":[{"name":"Institute of Earth Sciences Heidelberg University Heidelberg 69120 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Greiner","sequence":"additional","affiliation":[{"name":"Centre for Organismal Studies (COS) Im Neuenheimer Feld 360 Heidelberg 69120 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Klintzsch","sequence":"additional","affiliation":[{"name":"Institute of Earth Sciences Heidelberg University Heidelberg 69120 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2766-8812","authenticated-orcid":false,"given":"Frank","family":"Keppler","sequence":"additional","affiliation":[{"name":"Institute of Earth Sciences Heidelberg University Heidelberg 69120 Germany"},{"name":"Heidelberg Center for the Environment (HCE) Heidelberg University Heidelberg 69120 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2014.118"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atmosenv.2014.09.077"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s003740050510"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.82.3.718"},{"key":"e_1_2_9_6_1","volume-title":"Climate change 2007: the physical science basis. Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change","author":"Forster P","year":"2007"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1999.00494.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/abio.200390032"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.5194\/amt-13-2797-2020"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.5194\/amt-19-3557-2026"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9781107415324"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2009.02924.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.15455"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/gcb.12995"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep23410"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2024.173122"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.9296"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2005.01542.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/gcb.17181"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1176985"},{"key":"e_1_2_9_21_1","unstructured":"SchrollM MaasM GreinerS KlintzschT KepplerF.2026.Insights into plant\u2010part specific N2O production in roots and shoots of chicory (C. intybus) using stable isotope labelling.heiDATA. doi:10.11588\/DATA\/Y8B6MU."},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/pei3.10015"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.131572798"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.1.638-644.2006"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2780-0"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms231911522"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2021.150262"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2020.01177"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac9904563"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.21459"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.30.4.388"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/00031305.2019.1583913"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy14122875"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom13101443"}],"container-title":["New Phytologist"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nph.71565","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/nph.71565","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nph.71565","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T06:47:37Z","timestamp":1792046857000},"score":1,"resource":{"primary":{"URL":"https:\/\/nph.onlinelibrary.wiley.com\/doi\/10.1111\/nph.71565"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,15]]},"references-count":33,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["10.1111\/nph.71565"],"URL":"https:\/\/doi.org\/10.1111\/nph.71565","archive":["Portico"],"relation":{},"ISSN":["0028-646X","1469-8137"],"issn-type":[{"value":"0028-646X","type":"print"},{"value":"1469-8137","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,15]]},"assertion":[{"value":"2026-06-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}