{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T12:03:20Z","timestamp":1790683400587,"version":"4.1.0"},"reference-count":62,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000154","name":"Division of Integrative Organismal Systems","doi-asserted-by":"publisher","award":["IOS\u20101645659"],"award-info":[{"award-number":["IOS\u20101645659"]}],"id":[{"id":"10.13039\/100000154","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>Sensing of pathogen\u2010associated molecular patterns (PAMPs) by pattern recognition receptors at the cell surface initiates the first layer of host immunity against invading microbial pathogens. In plants, the immune receptor Flagellin Sensing 2 (FLS2) perceives bacterial flagellin to trigger downstream signaling that culminates in PAMP\u2010triggered immunity (PTI).<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          FLS2 protein levels are critical for effective PTI, yet the regulatory mechanisms governing its stability remain to be fully elucidated. Our previous work showed that the Arabidopsis Really Interesting Gene (RING) type ubiquitin ligase XBAT35.2 positively regulates plant immunity against bacterial pathogen\n                          <jats:italic>Pseudomonas syringae<\/jats:italic>\n                          pv\n                          <jats:italic>tomato<\/jats:italic>\n                          by promoting FLS2 stability.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          In this study, we demonstrate that XBAT35.2 interacts\n                          <jats:italic>in vivo<\/jats:italic>\n                          with two key ESCRT\u2010I complex components, VPS37\u20101 and VPS28\u20102. XBAT35.2 ubiquitinates VPS37\u20101 with K48\u2010linked polyubiquitin chains, leading to its proteasomal degradation, and modifies VPS28\u20102 with K63\u2010linked chains, reducing its interaction with FLS2. VPS37\u20101 and VPS28\u20102 play redundant, negative roles in FLS2\u2010mediated immunity by promoting vacuolar degradation of FLS2, displaying differential effects on distinct FLS2\u2010mediated immune responses. By targeting VPS37\u20101 and VPS28\u20102, XBAT35.2 intercepts this degradative pathway, thereby stabilizing FLS2 and enhancing host immunity.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>These findings uncover a novel regulatory circuit modulating FLS2 abundance and deepen our understanding of controlling the cell\u2010surface receptor homeostasis in plant immunity.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.71590","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:31:18Z","timestamp":1790681478000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Differential ubiquitin modifications of\n                    <scp>ESCRT<\/scp>\n                    \u2010I subunits\n                    <scp>VPS37<\/scp>\n                    \u20101 and\n                    <scp>VPS28<\/scp>\n                    \u20102 positively regulate plant immunity"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2158-4899","authenticated-orcid":false,"given":"Chaofeng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Plant Pathology, Center for Plant Science Innovation University of Nebraska  Lincoln NE 68588 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0739-2185","authenticated-orcid":false,"given":"Yi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, Center for Plant Science Innovation University of Nebraska  Lincoln NE 68588 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