{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:55:08Z","timestamp":1790751308242,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32270291"],"award-info":[{"award-number":["32270291"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32470797"],"award-info":[{"award-number":["32470797"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32500285"],"award-info":[{"award-number":["32500285"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32370329"],"award-info":[{"award-number":["32370329"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32470353"],"award-info":[{"award-number":["32470353"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2025YFA0921600"],"award-info":[{"award-number":["2025YFA0921600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2025YFA0921603"],"award-info":[{"award-number":["2025YFA0921603"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>Secretory proteins are essential for plant development and environmental adaptation, yet selectively profiling cargoes transported through the conventional endoplasmic reticulum (ER)\u2013Golgi pathway remains technically challenging because apoplastic secretomes contain proteins from multiple secretion routes.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          We developed an ER\u2010anchored TurboID proximity labeling method to selectively enrich proteins entering the conventional secretory pathway in\n                          <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                          . Quantitative proteomics under control and salt stress conditions identified dynamic secretome changes and showed that most enriched proteins carry N\u2010terminal signal peptides, supporting the utility of this approach for profiling conventional secretory proteins.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Using this method, we found that sodium chloride (NaCl) stress remodels the conventional secretome by enhancing the secretion of a subset of cell wall\u2010associated proteins. We further identified Apoplastic EDS1\u2010Dependent 3 (AED3) as a salt\u2010induced secretory protein, confirmed its ER\u2013Golgi\u2010dependent secretion, and demonstrated its role in salt tolerance.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Overall, this study provides a useful approach for pathway\u2010specific profiling of the conventional plant secretome, enabling the discovery of dynamic secretory proteins involved in plant environmental responses.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.71614","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:59:19Z","timestamp":1790747959000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Engineering a\n                    <scp>TurboID<\/scp>\n                    \u2010based proximity labeling tool to track the dynamic secretomes under salt stress"],"prefix":"10.1111","author":[{"given":"Danni","family":"Lin","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences South China Normal University  Guangzhou 510631 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Zheng","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences South China Normal University  Guangzhou 510631 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Song","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences South China Normal University  Guangzhou 510631 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