{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T03:49:32Z","timestamp":1791431372792,"version":"4.3.3"},"reference-count":92,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32100273"],"award-info":[{"award-number":["32100273"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004912","name":"Sichuan University","doi-asserted-by":"publisher","award":["0082604151432"],"award-info":[{"award-number":["0082604151432"]}],"id":[{"id":"10.13039\/501100004912","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100016102","name":"Organization Department of the Central Committee of the Communist Party of China","doi-asserted-by":"publisher","award":["SQ2024QB02593"],"award-info":[{"award-number":["SQ2024QB02593"]}],"id":[{"id":"10.13039\/501100016102","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>The ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT (ESCRT) proteins are essential for converting early endosomes (EEs) into late endosomes\/multivesicular bodies (LEs\/MVBs) during endocytosis. In animals, they also play noncanonical roles in cytokinetic abscission, membrane repair, and other membrane remodeling processes. However, their functions in plants, especially nonvascular plants, remain poorly understood.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Here, we systematically investigate the localization and functions of ESCRT proteins in the moss\n                          <jats:italic>Physcomitrium patens<\/jats:italic>\n                          using fluorescent protein labeling, mutant analyses, interaction assays, and electron microscopy.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          We show that ESCRT\u2010related proteins localize to endosomes and play a conserved role in endocytic trafficking. Notably, all tested ESCRT components also localize to the developing cell plate, whereas reporters involved in\n                          <jats:italic>trans<\/jats:italic>\n                          \u2010Golgi network (TGN)\u2010MVB transition do not. Disruption of VPS4, a key regulator of ESCRT\u2010III, delays cytokinesis progression and alters the dynamics of cell plate assembly factors.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>We propose that the ESCRT machinery plays both conserved and previously unrecognized noncanonical roles in plants. By providing this evidence in a bryophyte, our work establishes a critical reference point for future functional studies in nonvascular plants and for evolutionary comparisons across plant lineages.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.71651","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:53:06Z","timestamp":1791427986000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT<\/scp>\n                    machineries play crucial roles in vacuolar trafficking and cytokinesis in the moss\n                    <i>Physcomitrium patens<\/i>"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-9880-6446","authenticated-orcid":false,"given":"Jingtong","family":"Ruan","sequence":"first","affiliation":[{"name":"Key Laboratory of Bio\u2010Resource and Eco\u2010Environment of Ministry of Education, Laboratory for Ex Situ Conservation and Resource Utilization of Mountain Plants, College of Life Sciences Sichuan University  Chengdu 610065 Sichuan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8553-8577","authenticated-orcid":false,"given":"Zihan","family":"Yin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio\u2010Resource and Eco\u2010Environment of Ministry of Education, Laboratory for Ex Situ Conservation and Resource Utilization of Mountain Plants, College of Life Sciences Sichuan University  Chengdu 610065 Sichuan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5781-4186","authenticated-orcid":false,"given":"Yirong","family":"Gan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio\u2010Resource and Eco\u2010Environment of Ministry of Education, Laboratory for Ex Situ Conservation and Resource Utilization of Mountain Plants, College of Life Sciences Sichuan University  Chengdu 610065 Sichuan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7862-8511","authenticated-orcid":false,"given":"Lili","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio\u2010Resource and Eco\u2010Environment of Ministry of Education, Laboratory for Ex Situ Conservation and Resource Utilization of Mountain Plants, College of Life Sciences Sichuan University  Chengdu 610065 Sichuan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6358-4582","authenticated-orcid":false,"given":"Caiwan","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio\u2010Resource and Eco\u2010Environment of Ministry of Education, Laboratory for Ex Situ Conservation and Resource Utilization of Mountain Plants, College of Life Sciences Sichuan University  Chengdu 610065 Sichuan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4710-8089","authenticated-orcid":false,"given":"Peishan","family":"Yi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio\u2010Resource and Eco\u2010Environment of Ministry of Education, Laboratory for Ex Situ Conservation and Resource Utilization of Mountain Plants, College of Life Sciences Sichuan University  Chengdu 610065 Sichuan P.R. China"},{"name":"Chengdu Botanical Garden  Chengdu 610083 Sichuan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1083\/jcb.201310114","article-title":"Coordinated binding of Vps4 to ESCRT\u2010III drives membrane neck constriction during MVB vesicle formation","volume":"205","author":"Adell MA","year":"2014","journal-title":"Journal of Cell Biology"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1105\/tpc.17.00108","article-title":"SH3 domain\u2010containing protein 2 plays a crucial role at the step of membrane tubulation during cell plate formation","volume":"29","author":"Ahn G","year":"2017","journal-title":"Plant Cell"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1093\/plphys\/kiab077","article-title":"Motif\u2010based endomembrane trafficking","volume":"186","author":"Arora 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