{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T11:56:31Z","timestamp":1792151791891,"version":"4.4.0"},"reference-count":34,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004189","name":"Max-Planck-Gesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004189","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005713","name":"Technische Universit\u00e4t M\u00fcnchen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005713","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"publisher","award":["759731"],"award-info":[{"award-number":["759731"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"abstract":"Summary<\/jats:title>\n \n \n \n \n Arbuscular mycorrhiza (AM), a symbiosis between plants and Glomeromycotan fungi is based on the exchange of nutrients, which majorly occurs between fungal tree\u2010shaped arbuscules and root cortex cells. Two homologous plant GRAS transcription factors, RAM1 and RAD1, were previously reported to interact, and both were implicated in regulating arbuscule branching in\n Lotus japonicus<\/jats:italic>\n . We revisited the role of\n L. japonicus RAM1<\/jats:italic>\n and\n RAD1<\/jats:italic>\n and asked whether they act together in mediating arbuscule development and gene expression, or whether they act additively.\n <\/jats:p>\n <\/jats:list-item>\n \n \n We performed comprehensive genetic, phenotypic, and transcriptome analyses of two allelic\n Lotus ram1<\/jats:italic>\n ,\n rad1<\/jats:italic>\n , and\n ram1 rad1<\/jats:italic>\n mutants; and of roots ectopically expressing RAM1 and\/or RAD1.\n <\/jats:p>\n <\/jats:list-item>\n \n \n We found that\n RAD1<\/jats:italic>\n is required for full root colonization in a\n RAM1<\/jats:italic>\n \u2010dependent manner, but in our growth conditions,\n RAD1<\/jats:italic>\n is not required for arbuscule branching. In addition,\n RAM1<\/jats:italic>\n and\n RAD1<\/jats:italic>\n are required for activation of partially common and partially distinct sets of downstream genes.\n <\/jats:p>\n <\/jats:list-item>\n \n \n Thus, in contrast to\n RAM1<\/jats:italic>\n ,\n Lotus RAD1<\/jats:italic>\n is not essential for arbuscule branching. Furthermore, RAM1 and RAD1 seem to act partially synergistically but seem to participate in additional alternative transcription factor complexes to regulate AM\u2010induced gene expression and AM development.\n <\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>","DOI":"10.1111\/nph.71662","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T11:31:11Z","timestamp":1792150271000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The homologous\n GRAS<\/scp>\n transcription factors\n RAM1<\/scp>\n and\n RAD1<\/scp>\n coordinate shared and separate gene networks to regulate arbuscular mycorrhiza symbiosis"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0315-7658","authenticated-orcid":false,"given":"Tian","family":"Zeng","sequence":"first","affiliation":[{"name":"Max Planck Institute of Molecular Plant Physiology 14476 Potsdam\u2010Golm Germany"},{"name":"Plant Genetics, TUM School of Life Sciences Technical University of Munich 85354 Freising Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Alpers","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Molecular Plant Physiology 14476 Potsdam\u2010Golm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Boniolo","sequence":"additional","affiliation":[{"name":"Data Science in Systems Biology, School of Life Sciences Technical University of Munich 85354 Freising Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regine","family":"Huettl","sequence":"additional","affiliation":[{"name":"Plant Genetics, TUM School of Life Sciences Technical University of Munich 85354 Freising Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5268-4276","authenticated-orcid":false,"given":"Florian","family":"Berger","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Molecular Plant Physiology 14476 Potsdam\u2010Golm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Hernandez Cortes","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Molecular Plant Physiology 14476 Potsdam\u2010Golm Germany"},{"name":"Plant Genetics, TUM School of Life Sciences Technical University of Munich 85354 Freising Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6163-745X","authenticated-orcid":false,"given":"Caroline","family":"Gutjahr","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Molecular Plant Physiology 14476 Potsdam\u2010Golm Germany"},{"name":"Plant Genetics, TUM School of Life Sciences Technical University of Munich 85354 Freising Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/nplants.2015.208","article-title":"Genes conserved for arbuscular mycorrhizal symbiosis identified through phylogenomics","volume":"2","author":"Bravo A","year":"2016","journal-title":"Nature Plants"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1111\/nph.14913","article-title":"Glomeromycotina: what is a species and why should we care?","volume":"220","author":"Bruns TD","year":"2018","journal-title":"New Phytologist"},{"key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1111\/nph.14989","article-title":"High intraspecific genome diversity in the model arbuscular mycorrhizal symbiont Rhizophagus irregularis","volume":"220","author":"Chen 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